Internal Subdivisions of the Marmoset Claustrum Complex: Identification by Myeloarchitectural Features and High Field Strength Imaging

Xiuxian Pham, David K. Wright, Nafiseh Atapour, Jonathan M.-H. Chan, Kirsty J. Watkins, Katrina H. Worthy, Marcello Rosa, Amy Reichelt, David H. Reser

Research output: Contribution to journalArticleResearchpeer-review

Abstract

There has been a surge of interest in the structure and function of the mammalian claustrum in recent years. However, most anatomical and physiological studies treat the claustrum as a relatively homogenous structure. Relatively little attention has been directed toward possible compartmentalization of the claustrum complex into anatomical subdivisions, and how this compartmentalization is reflected in claustrum connections with other brain structures. In this study, we examined the cyto- and myelo-architecture of the claustrum of the common marmoset (Callithrix jacchus), to determine whether the claustrum contains internal anatomical structures or compartments, which could facilitate studies focused on understanding its role in brain function. NeuN, Nissl, calbindin, parvalbumin, and myelin-stained sections from eight adult marmosets were studied using light microscopy and serial reconstruction to identify potential internal compartments. Ultra high resolution (9.4T) post-mortem magnetic resonance imaging was employed to identify tractographic differences between identified claustrum subcompartments by diffusion-weighted tractography. Our results indicate that the classically defined marmoset claustrum includes at least two major subdivisions, which correspond to the dorsal endopiriform and insular claustrum nuclei, as described in other species, and that the dorsal endopiriform nucleus (DEnD) contains architecturally distinct compartments. Furthermore, the dorsal subdivision of the DEnD is tractographically distinguishable from the insular claustrum with respect to cortical connections.

Original languageEnglish
Article number96
Number of pages13
JournalFrontiers in Neuroanatomy
Volume13
DOIs
Publication statusPublished - Nov 2019

Keywords

  • claustrum
  • forebrain and brainstem afferent pathways
  • marmoset
  • myelin
  • myeloarchitectonics
  • non-human primate

Cite this

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title = "Internal Subdivisions of the Marmoset Claustrum Complex: Identification by Myeloarchitectural Features and High Field Strength Imaging",
abstract = "There has been a surge of interest in the structure and function of the mammalian claustrum in recent years. However, most anatomical and physiological studies treat the claustrum as a relatively homogenous structure. Relatively little attention has been directed toward possible compartmentalization of the claustrum complex into anatomical subdivisions, and how this compartmentalization is reflected in claustrum connections with other brain structures. In this study, we examined the cyto- and myelo-architecture of the claustrum of the common marmoset (Callithrix jacchus), to determine whether the claustrum contains internal anatomical structures or compartments, which could facilitate studies focused on understanding its role in brain function. NeuN, Nissl, calbindin, parvalbumin, and myelin-stained sections from eight adult marmosets were studied using light microscopy and serial reconstruction to identify potential internal compartments. Ultra high resolution (9.4T) post-mortem magnetic resonance imaging was employed to identify tractographic differences between identified claustrum subcompartments by diffusion-weighted tractography. Our results indicate that the classically defined marmoset claustrum includes at least two major subdivisions, which correspond to the dorsal endopiriform and insular claustrum nuclei, as described in other species, and that the dorsal endopiriform nucleus (DEnD) contains architecturally distinct compartments. Furthermore, the dorsal subdivision of the DEnD is tractographically distinguishable from the insular claustrum with respect to cortical connections.",
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author = "Xiuxian Pham and Wright, {David K.} and Nafiseh Atapour and Chan, {Jonathan M.-H.} and Watkins, {Kirsty J.} and Worthy, {Katrina H.} and Marcello Rosa and Amy Reichelt and Reser, {David H.}",
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Internal Subdivisions of the Marmoset Claustrum Complex : Identification by Myeloarchitectural Features and High Field Strength Imaging. / Pham, Xiuxian; Wright, David K.; Atapour, Nafiseh; Chan, Jonathan M.-H.; Watkins, Kirsty J.; Worthy, Katrina H.; Rosa, Marcello; Reichelt, Amy; Reser, David H.

In: Frontiers in Neuroanatomy, Vol. 13, 96, 11.2019.

Research output: Contribution to journalArticleResearchpeer-review

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T2 - Identification by Myeloarchitectural Features and High Field Strength Imaging

AU - Pham, Xiuxian

AU - Wright, David K.

AU - Atapour, Nafiseh

AU - Chan, Jonathan M.-H.

AU - Watkins, Kirsty J.

AU - Worthy, Katrina H.

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AU - Reichelt, Amy

AU - Reser, David H.

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KW - claustrum

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KW - myelin

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