Interleukin-27 inhibits ectopic lymphoid-like structure development in early inflammatory arthritis

Gareth Wyn Jones, Michele Bombardieri, Claire J Greenhill, Louise McLeod, Alessandra Nerviani, Vidalba Rocher-Ros, Anna Cardus, Anwen S Williams, Constantino Pitzalis, Brendan John Jenkins, Simon A Jones

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Abstract

Ectopic lymphoid-like structures (ELSs) reminiscent of secondary lymphoid organs often develop at sites of chronic inflammation where they contribute to immune-mediated pathology. Through evaluation of synovial tissues from rheumatoid arthritis (RA) patients, we now show that low interleukin-27 (IL-27) expression corresponds with an increased incidence of ELS and gene signatures associated with their development and activity. The presence of synovial ELS was also noted in mice deficient in the IL-27 receptor (IL-27R) after the onset of inflammatory arthritis. Here, pathology was associated with increased synovial expression of pro-inflammatory cytokines, homeostatic chemokines, and transcriptional regulators linked with lymphoid neogenesis. In both clinical and experimental RA, synovial ELS coincided with the heightened local expression of cytokines and transcription factors of the Th17 and T follicular helper (Tfh) cell lineages, and included podoplanin-expressing T cells within lymphoid aggregates. IL-27 inhibited the differentiation of podoplanin-expressing Th17 cells, and an increased number of these cells were observed in IL-27R-deficient mice with inflammatory arthritis. Thus, IL-27 appears to negatively regulate ELS development in RA through control of effector T cells. These studies open new opportunities for patient stratification and treatment.
Original languageEnglish
Pages (from-to)1793 - 1802
Number of pages10
JournalJournal of Experimental Medicine
Volume212
Issue number11
DOIs
Publication statusPublished - 2015

Cite this

Jones, G. W., Bombardieri, M., Greenhill, C. J., McLeod, L., Nerviani, A., Rocher-Ros, V., ... Jones, S. A. (2015). Interleukin-27 inhibits ectopic lymphoid-like structure development in early inflammatory arthritis. Journal of Experimental Medicine, 212(11), 1793 - 1802. https://doi.org/10.1084/jem.20132307
Jones, Gareth Wyn ; Bombardieri, Michele ; Greenhill, Claire J ; McLeod, Louise ; Nerviani, Alessandra ; Rocher-Ros, Vidalba ; Cardus, Anna ; Williams, Anwen S ; Pitzalis, Constantino ; Jenkins, Brendan John ; Jones, Simon A. / Interleukin-27 inhibits ectopic lymphoid-like structure development in early inflammatory arthritis. In: Journal of Experimental Medicine. 2015 ; Vol. 212, No. 11. pp. 1793 - 1802.
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abstract = "Ectopic lymphoid-like structures (ELSs) reminiscent of secondary lymphoid organs often develop at sites of chronic inflammation where they contribute to immune-mediated pathology. Through evaluation of synovial tissues from rheumatoid arthritis (RA) patients, we now show that low interleukin-27 (IL-27) expression corresponds with an increased incidence of ELS and gene signatures associated with their development and activity. The presence of synovial ELS was also noted in mice deficient in the IL-27 receptor (IL-27R) after the onset of inflammatory arthritis. Here, pathology was associated with increased synovial expression of pro-inflammatory cytokines, homeostatic chemokines, and transcriptional regulators linked with lymphoid neogenesis. In both clinical and experimental RA, synovial ELS coincided with the heightened local expression of cytokines and transcription factors of the Th17 and T follicular helper (Tfh) cell lineages, and included podoplanin-expressing T cells within lymphoid aggregates. IL-27 inhibited the differentiation of podoplanin-expressing Th17 cells, and an increased number of these cells were observed in IL-27R-deficient mice with inflammatory arthritis. Thus, IL-27 appears to negatively regulate ELS development in RA through control of effector T cells. These studies open new opportunities for patient stratification and treatment.",
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Jones, GW, Bombardieri, M, Greenhill, CJ, McLeod, L, Nerviani, A, Rocher-Ros, V, Cardus, A, Williams, AS, Pitzalis, C, Jenkins, BJ & Jones, SA 2015, 'Interleukin-27 inhibits ectopic lymphoid-like structure development in early inflammatory arthritis', Journal of Experimental Medicine, vol. 212, no. 11, pp. 1793 - 1802. https://doi.org/10.1084/jem.20132307

Interleukin-27 inhibits ectopic lymphoid-like structure development in early inflammatory arthritis. / Jones, Gareth Wyn; Bombardieri, Michele; Greenhill, Claire J; McLeod, Louise; Nerviani, Alessandra; Rocher-Ros, Vidalba; Cardus, Anna; Williams, Anwen S; Pitzalis, Constantino; Jenkins, Brendan John; Jones, Simon A.

In: Journal of Experimental Medicine, Vol. 212, No. 11, 2015, p. 1793 - 1802.

Research output: Contribution to journalArticleResearchpeer-review

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AU - Jones, Gareth Wyn

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AU - McLeod, Louise

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AU - Rocher-Ros, Vidalba

AU - Cardus, Anna

AU - Williams, Anwen S

AU - Pitzalis, Constantino

AU - Jenkins, Brendan John

AU - Jones, Simon A

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N2 - Ectopic lymphoid-like structures (ELSs) reminiscent of secondary lymphoid organs often develop at sites of chronic inflammation where they contribute to immune-mediated pathology. Through evaluation of synovial tissues from rheumatoid arthritis (RA) patients, we now show that low interleukin-27 (IL-27) expression corresponds with an increased incidence of ELS and gene signatures associated with their development and activity. The presence of synovial ELS was also noted in mice deficient in the IL-27 receptor (IL-27R) after the onset of inflammatory arthritis. Here, pathology was associated with increased synovial expression of pro-inflammatory cytokines, homeostatic chemokines, and transcriptional regulators linked with lymphoid neogenesis. In both clinical and experimental RA, synovial ELS coincided with the heightened local expression of cytokines and transcription factors of the Th17 and T follicular helper (Tfh) cell lineages, and included podoplanin-expressing T cells within lymphoid aggregates. IL-27 inhibited the differentiation of podoplanin-expressing Th17 cells, and an increased number of these cells were observed in IL-27R-deficient mice with inflammatory arthritis. Thus, IL-27 appears to negatively regulate ELS development in RA through control of effector T cells. These studies open new opportunities for patient stratification and treatment.

AB - Ectopic lymphoid-like structures (ELSs) reminiscent of secondary lymphoid organs often develop at sites of chronic inflammation where they contribute to immune-mediated pathology. Through evaluation of synovial tissues from rheumatoid arthritis (RA) patients, we now show that low interleukin-27 (IL-27) expression corresponds with an increased incidence of ELS and gene signatures associated with their development and activity. The presence of synovial ELS was also noted in mice deficient in the IL-27 receptor (IL-27R) after the onset of inflammatory arthritis. Here, pathology was associated with increased synovial expression of pro-inflammatory cytokines, homeostatic chemokines, and transcriptional regulators linked with lymphoid neogenesis. In both clinical and experimental RA, synovial ELS coincided with the heightened local expression of cytokines and transcription factors of the Th17 and T follicular helper (Tfh) cell lineages, and included podoplanin-expressing T cells within lymphoid aggregates. IL-27 inhibited the differentiation of podoplanin-expressing Th17 cells, and an increased number of these cells were observed in IL-27R-deficient mice with inflammatory arthritis. Thus, IL-27 appears to negatively regulate ELS development in RA through control of effector T cells. These studies open new opportunities for patient stratification and treatment.

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