Interleukin-17A differentially modulates BCG induction of cytokine production in human blood macrophages

Jun-wei Fang, James Li, Au Kin-yi, Howard Yim, Allan Lau

Research output: Contribution to journalArticleResearchpeer-review

11 Citations (Scopus)

Abstract

The pathogenesis of Mtb depends in part on cytokine cross-regulation between macrophages and T cells in host immunity. Th17 cells produce IL-17A to induce granuloma formation and to restrict mycobacterial dissemination. IL-17A also mediates cytokine responses induced by proinflammatory cytokines such as TNF-alpha. Our previous results showed that BCG induces IL-6, IL-10, and TNF-alpha via activity of protein kinases, including dsRNA-activated serine/threonine protein kinase and glycogen synthase kinase-3 in primary human monocytes. Therefore, we investigated whether IL-17A, upon its induction by BCG, plays an additional role to aid the production of downstream proinflammatory cytokines in macrophages. Here, we showed that IL-17A enhanced IL-6 mRNA and protein levels inducible by BCG in a time- and dose-dependent manner, whereas it had no effect on IL-10 and TNF-alpha production. We also demonstrated that IL-17A activated the phosphorylation of ERK1/2 triggered by BCG. With the use of a specific chemical inhibitor of a MAPK/ERK-activating kinase (MEK1/2), we confirmed the correlation between the enhanced ERK1/2 activation and augmented IL-6 production. Additionally, we revealed that IL-17A acts in concert with BCG-induced TNF-alpha to enhance the level of IL-6 synthesis. Taken together, our results suggest a significant role of IL-17A to serve as a modulator of cytokine expression in innate immune response during mycobacterial infection.
Original languageEnglish
Pages (from-to)333 - 341
Number of pages9
JournalJournal of leukocyte biology
Volume90
Issue number2
DOIs
Publication statusPublished - 2011
Externally publishedYes

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