Intergenerational Prevention Of Heart Failure Through Maternal Intake Of High Fibre

Hamdi Ahmed Jama, Waled Shihata, Mark Daniel Ziemann, Daniel G Donner, Helen Kiriazis, Xiao-Jun Du, Assam El-Osta, Charles R. Mackay, David Martin Kaye, Francine Coelho Marques

Research output: Contribution to conferenceAbstractOther

Abstract

Background/Aims: Dietary fibre intake protects against the development of cardiovascular disease (CVD) through the production of gut microbial metabolites. We aim to determine whether dietary fibre during pregnancy can prevent the development heart failure through changes in the gut microbiota using the angiotensin II (Ang II) minipump model of hypertension Method: C57BL/6 female mice were fed a diet without (‘no fibre’) or with high resistant starches (‘high fibre’) during gestation. At 6-weeks of age, male offspring had minipumps containing saline or Ang II (0.25mg/kg/day) subcutaneously implanted. Mice were followed for 4-weeks and cardiac weight, gene expression and gut microbiome composition were determined. 2-way ANOVA with adjustment for multiple comparison was used and P<0.05 was considered significant. Results: Mothers fed diets without or with high levels of fibre had a different gut microbiome composition (P=0.001). Pups born from high fibre mothers had distinct microbial colonisation (P=0.001), irrespective of the presence of Ang II (P=0.013). Independently of the mothers’ diet, Ang II mice had higher systolic and diastolic blood pressure, but no difference in weight gain. Ang II offspring from high fibre mothers had a significant decrease in heart to body weight ratio (P=0.034). Furthermore, Ang II offspring whose mothers received a high fibre diet had lower levels of markers of fibrosis such as Col1a1 (P=0.01), Tgfb (P=0.021) and Ctgf mRNA (P=0.005) and lower levels of Nppb mRNA, a marker of cardiac remodelling and heart failure (P=0.002). Conclusions: High fibre intake during pregnancy protected the offspring against the development of cardiac fibrosis and hypertrophy when compared to those born from mothers who consumed no fibre. The gut microbiota of the mothers was shaped by the intake of fibre during pregnancy and this had a lasting founding effect in the offsprings’ microbiota.
Original languageEnglish
Publication statusPublished - Feb 2019
EventDOHaD 2019 - Melbourne Convention Centre, South Wharf, Australia
Duration: 20 Oct 201923 Oct 2019
http://www.cvent.com/events/2019-dohad-international/event-summary-828d23c3caf043c0ae6449de8d7bfbc3.aspx

Conference

ConferenceDOHaD 2019
CountryAustralia
CitySouth Wharf
Period20/10/1923/10/19
Internet address

Cite this

Jama, H. A., Shihata, W., Ziemann, M. D., Donner, D. G., Kiriazis, H., Du, X-J., ... Coelho Marques, F. (2019). Intergenerational Prevention Of Heart Failure Through Maternal Intake Of High Fibre. Abstract from DOHaD 2019, South Wharf, Australia.
Jama, Hamdi Ahmed ; Shihata, Waled ; Ziemann, Mark Daniel ; Donner, Daniel G ; Kiriazis, Helen ; Du, Xiao-Jun ; El-Osta, Assam ; Mackay, Charles R. ; Kaye, David Martin ; Coelho Marques, Francine. / Intergenerational Prevention Of Heart Failure Through Maternal Intake Of High Fibre. Abstract from DOHaD 2019, South Wharf, Australia.
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title = "Intergenerational Prevention Of Heart Failure Through Maternal Intake Of High Fibre",
abstract = "Background/Aims: Dietary fibre intake protects against the development of cardiovascular disease (CVD) through the production of gut microbial metabolites. We aim to determine whether dietary fibre during pregnancy can prevent the development heart failure through changes in the gut microbiota using the angiotensin II (Ang II) minipump model of hypertension Method: C57BL/6 female mice were fed a diet without (‘no fibre’) or with high resistant starches (‘high fibre’) during gestation. At 6-weeks of age, male offspring had minipumps containing saline or Ang II (0.25mg/kg/day) subcutaneously implanted. Mice were followed for 4-weeks and cardiac weight, gene expression and gut microbiome composition were determined. 2-way ANOVA with adjustment for multiple comparison was used and P<0.05 was considered significant. Results: Mothers fed diets without or with high levels of fibre had a different gut microbiome composition (P=0.001). Pups born from high fibre mothers had distinct microbial colonisation (P=0.001), irrespective of the presence of Ang II (P=0.013). Independently of the mothers’ diet, Ang II mice had higher systolic and diastolic blood pressure, but no difference in weight gain. Ang II offspring from high fibre mothers had a significant decrease in heart to body weight ratio (P=0.034). Furthermore, Ang II offspring whose mothers received a high fibre diet had lower levels of markers of fibrosis such as Col1a1 (P=0.01), Tgfb (P=0.021) and Ctgf mRNA (P=0.005) and lower levels of Nppb mRNA, a marker of cardiac remodelling and heart failure (P=0.002). Conclusions: High fibre intake during pregnancy protected the offspring against the development of cardiac fibrosis and hypertrophy when compared to those born from mothers who consumed no fibre. The gut microbiota of the mothers was shaped by the intake of fibre during pregnancy and this had a lasting founding effect in the offsprings’ microbiota.",
author = "Jama, {Hamdi Ahmed} and Waled Shihata and Ziemann, {Mark Daniel} and Donner, {Daniel G} and Helen Kiriazis and Xiao-Jun Du and Assam El-Osta and Mackay, {Charles R.} and Kaye, {David Martin} and {Coelho Marques}, Francine",
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language = "English",
note = "DOHaD 2019 ; Conference date: 20-10-2019 Through 23-10-2019",
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Jama, HA, Shihata, W, Ziemann, MD, Donner, DG, Kiriazis, H, Du, X-J, El-Osta, A, Mackay, CR, Kaye, DM & Coelho Marques, F 2019, 'Intergenerational Prevention Of Heart Failure Through Maternal Intake Of High Fibre' DOHaD 2019, South Wharf, Australia, 20/10/19 - 23/10/19, .

Intergenerational Prevention Of Heart Failure Through Maternal Intake Of High Fibre. / Jama, Hamdi Ahmed; Shihata, Waled; Ziemann, Mark Daniel; Donner, Daniel G; Kiriazis, Helen; Du, Xiao-Jun; El-Osta, Assam; Mackay, Charles R.; Kaye, David Martin; Coelho Marques, Francine.

2019. Abstract from DOHaD 2019, South Wharf, Australia.

Research output: Contribution to conferenceAbstractOther

TY - CONF

T1 - Intergenerational Prevention Of Heart Failure Through Maternal Intake Of High Fibre

AU - Jama, Hamdi Ahmed

AU - Shihata, Waled

AU - Ziemann, Mark Daniel

AU - Donner, Daniel G

AU - Kiriazis, Helen

AU - Du, Xiao-Jun

AU - El-Osta, Assam

AU - Mackay, Charles R.

AU - Kaye, David Martin

AU - Coelho Marques, Francine

PY - 2019/2

Y1 - 2019/2

N2 - Background/Aims: Dietary fibre intake protects against the development of cardiovascular disease (CVD) through the production of gut microbial metabolites. We aim to determine whether dietary fibre during pregnancy can prevent the development heart failure through changes in the gut microbiota using the angiotensin II (Ang II) minipump model of hypertension Method: C57BL/6 female mice were fed a diet without (‘no fibre’) or with high resistant starches (‘high fibre’) during gestation. At 6-weeks of age, male offspring had minipumps containing saline or Ang II (0.25mg/kg/day) subcutaneously implanted. Mice were followed for 4-weeks and cardiac weight, gene expression and gut microbiome composition were determined. 2-way ANOVA with adjustment for multiple comparison was used and P<0.05 was considered significant. Results: Mothers fed diets without or with high levels of fibre had a different gut microbiome composition (P=0.001). Pups born from high fibre mothers had distinct microbial colonisation (P=0.001), irrespective of the presence of Ang II (P=0.013). Independently of the mothers’ diet, Ang II mice had higher systolic and diastolic blood pressure, but no difference in weight gain. Ang II offspring from high fibre mothers had a significant decrease in heart to body weight ratio (P=0.034). Furthermore, Ang II offspring whose mothers received a high fibre diet had lower levels of markers of fibrosis such as Col1a1 (P=0.01), Tgfb (P=0.021) and Ctgf mRNA (P=0.005) and lower levels of Nppb mRNA, a marker of cardiac remodelling and heart failure (P=0.002). Conclusions: High fibre intake during pregnancy protected the offspring against the development of cardiac fibrosis and hypertrophy when compared to those born from mothers who consumed no fibre. The gut microbiota of the mothers was shaped by the intake of fibre during pregnancy and this had a lasting founding effect in the offsprings’ microbiota.

AB - Background/Aims: Dietary fibre intake protects against the development of cardiovascular disease (CVD) through the production of gut microbial metabolites. We aim to determine whether dietary fibre during pregnancy can prevent the development heart failure through changes in the gut microbiota using the angiotensin II (Ang II) minipump model of hypertension Method: C57BL/6 female mice were fed a diet without (‘no fibre’) or with high resistant starches (‘high fibre’) during gestation. At 6-weeks of age, male offspring had minipumps containing saline or Ang II (0.25mg/kg/day) subcutaneously implanted. Mice were followed for 4-weeks and cardiac weight, gene expression and gut microbiome composition were determined. 2-way ANOVA with adjustment for multiple comparison was used and P<0.05 was considered significant. Results: Mothers fed diets without or with high levels of fibre had a different gut microbiome composition (P=0.001). Pups born from high fibre mothers had distinct microbial colonisation (P=0.001), irrespective of the presence of Ang II (P=0.013). Independently of the mothers’ diet, Ang II mice had higher systolic and diastolic blood pressure, but no difference in weight gain. Ang II offspring from high fibre mothers had a significant decrease in heart to body weight ratio (P=0.034). Furthermore, Ang II offspring whose mothers received a high fibre diet had lower levels of markers of fibrosis such as Col1a1 (P=0.01), Tgfb (P=0.021) and Ctgf mRNA (P=0.005) and lower levels of Nppb mRNA, a marker of cardiac remodelling and heart failure (P=0.002). Conclusions: High fibre intake during pregnancy protected the offspring against the development of cardiac fibrosis and hypertrophy when compared to those born from mothers who consumed no fibre. The gut microbiota of the mothers was shaped by the intake of fibre during pregnancy and this had a lasting founding effect in the offsprings’ microbiota.

M3 - Abstract

ER -

Jama HA, Shihata W, Ziemann MD, Donner DG, Kiriazis H, Du X-J et al. Intergenerational Prevention Of Heart Failure Through Maternal Intake Of High Fibre. 2019. Abstract from DOHaD 2019, South Wharf, Australia.