Abstract
| Original language | English |
|---|---|
| Pages (from-to) | 478-483 |
| Number of pages | 6 |
| Journal | Immunology and Cell Biology |
| Volume | 95 |
| Issue number | 5 |
| DOIs | |
| Publication status | Published - May 2017 |
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Interferon epsilon promotes HIV restriction at multiple steps of viral replication. / Garcia-Minambres, Albert; Eid, Sahar G; Mangan, Niamh E.; Pade, Corinna; Lim, San S; Matthews, Antony Y; de Weerd, Nicole A.; Hertzog, Paul J.; Johnson, Mak.
In: Immunology and Cell Biology, Vol. 95, No. 5, 05.2017, p. 478-483.Research output: Contribution to journal › Article › Research › peer-review
TY - JOUR
T1 - Interferon epsilon promotes HIV restriction at multiple steps of viral replication
AU - Garcia-Minambres, Albert
AU - Eid, Sahar G
AU - Mangan, Niamh E.
AU - Pade, Corinna
AU - Lim, San S
AU - Matthews, Antony Y
AU - de Weerd, Nicole A.
AU - Hertzog, Paul J.
AU - Johnson, Mak
PY - 2017/5
Y1 - 2017/5
N2 - Interferon epsilon (IFNɛ) is a type I IFN that is expressed constitutively in the female reproductive tract (FRT), and contributes to protection in models of sexually transmitted infections. Using multiple cell systems, including reporter cell lines and activated peripheral blood lymphocytes (PBLs), we show that recombinant IFNɛ impairs HIV infection at stage(s) post HIV entry and up to the translation of viral proteins. Consistent with this, IFNɛ upregulated a number of host cell restriction factors that block HIV at these stages of the replication cycle. The potency of IFNɛ induction of these HIV restriction factors was comparable to conventional type I IFNs, namely IFNα and IFNβ. IFNɛ also significantly reduced the infectivity of progeny virion particles likely by inducing expression of HIV restriction factors, such as IFITM3, which act at that stage of infection. Thus, our data demonstrate that human IFNɛ suppresses HIV replication at multiple stages of infection.Immunology and Cell Biology advance online publication, 3 January 2017; doi:10.1038/icb.2016.123.
AB - Interferon epsilon (IFNɛ) is a type I IFN that is expressed constitutively in the female reproductive tract (FRT), and contributes to protection in models of sexually transmitted infections. Using multiple cell systems, including reporter cell lines and activated peripheral blood lymphocytes (PBLs), we show that recombinant IFNɛ impairs HIV infection at stage(s) post HIV entry and up to the translation of viral proteins. Consistent with this, IFNɛ upregulated a number of host cell restriction factors that block HIV at these stages of the replication cycle. The potency of IFNɛ induction of these HIV restriction factors was comparable to conventional type I IFNs, namely IFNα and IFNβ. IFNɛ also significantly reduced the infectivity of progeny virion particles likely by inducing expression of HIV restriction factors, such as IFITM3, which act at that stage of infection. Thus, our data demonstrate that human IFNɛ suppresses HIV replication at multiple stages of infection.Immunology and Cell Biology advance online publication, 3 January 2017; doi:10.1038/icb.2016.123.
UR - http://www.scopus.com/inward/record.url?scp=85008354094&partnerID=8YFLogxK
U2 - 10.1038/icb.2016.123
DO - 10.1038/icb.2016.123
M3 - Article
VL - 95
SP - 478
EP - 483
JO - Immunology and Cell Biology
JF - Immunology and Cell Biology
SN - 0818-9641
IS - 5
ER -