Interferon γ is recognised by importin  α/β: enhanced nuclear localising and transactivation activities of an interferon gamma mimetic

Alex Fulcher; Chulbul M I Ahmed; Ezra N Noon-Song; Rain Y Q Kwan; Prem S Subramaniam; Howard M Johnson; David Andrew Jans

doi:10.1016/j.febslet.2008.03.054

Interferon γ is recognised by importin α/β: enhanced nuclear localising and transactivation activities of an interferon gamma mimetic

Alex Fulcher, Chulbul M I Ahmed, Ezra N Noon-Song, Rain Y Q Kwan, Prem S Subramaniam, Howard M Johnson, David Andrew Jans

Biochemistry & Molecular Biology

Research output: Contribution to journal › Article › Research › peer-review

4 Citations (Scopus)

Abstract

Interferon (INF) gamma s ability to localise in the nucleus and function in gene activation has been known for some time, although the role of the conventional nuclear transporting importin molecules is unclear. Here, we demonstrate for the first time the direct recognition of IFNgamma and an IFNgamma mimetic peptide by IMPalpha and the IMPalpha/beta heterodimer, where the IFNgamma mimetic shows higher affinity. Significantly, this correlates well both with in vivo ability to target green fluorescent protein to the nucleus in transfected cells as determined by quantitative confocal laser scanning microscopy, as well as GAS promoter activity of a luciferase reporter. This has important implications for IFNgamma s anti-viral action, and the potential use of the IFNgamma mimetic in antiviral therapies. STRUCTURED SUMMARY:

Original language	English
Pages (from-to)	1569 - 1574
Number of pages	6
Journal	FEBS Letters
Volume	582
Issue number	11
DOIs	https://doi.org/10.1016/j.febslet.2008.03.054
Publication status	Published - 2008

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10.1016/j.febslet.2008.03.054

Cite this

@article{2fc2b849b3bb47498513f4a24dcf5593,

title = "Interferon γ is recognised by importin α/β: enhanced nuclear localising and transactivation activities of an interferon gamma mimetic",

abstract = "Interferon (INF) gamma s ability to localise in the nucleus and function in gene activation has been known for some time, although the role of the conventional nuclear transporting importin molecules is unclear. Here, we demonstrate for the first time the direct recognition of IFNgamma and an IFNgamma mimetic peptide by IMPalpha and the IMPalpha/beta heterodimer, where the IFNgamma mimetic shows higher affinity. Significantly, this correlates well both with in vivo ability to target green fluorescent protein to the nucleus in transfected cells as determined by quantitative confocal laser scanning microscopy, as well as GAS promoter activity of a luciferase reporter. This has important implications for IFNgamma s anti-viral action, and the potential use of the IFNgamma mimetic in antiviral therapies. STRUCTURED SUMMARY:",

author = "Alex Fulcher and Ahmed, {Chulbul M I} and Noon-Song, {Ezra N} and Kwan, {Rain Y Q} and Subramaniam, {Prem S} and Johnson, {Howard M} and Jans, {David Andrew}",

year = "2008",

doi = "10.1016/j.febslet.2008.03.054",

language = "English",

volume = "582",

pages = "1569 -- 1574",

journal = "FEBS Letters",

issn = "0014-5793",

publisher = "John Wiley & Sons",

number = "11",

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TY - JOUR

T1 - Interferon γ is recognised by importin α/β

T2 - enhanced nuclear localising and transactivation activities of an interferon gamma mimetic

AU - Fulcher, Alex

AU - Ahmed, Chulbul M I

AU - Noon-Song, Ezra N

AU - Kwan, Rain Y Q

AU - Subramaniam, Prem S

AU - Johnson, Howard M

AU - Jans, David Andrew

PY - 2008

Y1 - 2008

N2 - Interferon (INF) gamma s ability to localise in the nucleus and function in gene activation has been known for some time, although the role of the conventional nuclear transporting importin molecules is unclear. Here, we demonstrate for the first time the direct recognition of IFNgamma and an IFNgamma mimetic peptide by IMPalpha and the IMPalpha/beta heterodimer, where the IFNgamma mimetic shows higher affinity. Significantly, this correlates well both with in vivo ability to target green fluorescent protein to the nucleus in transfected cells as determined by quantitative confocal laser scanning microscopy, as well as GAS promoter activity of a luciferase reporter. This has important implications for IFNgamma s anti-viral action, and the potential use of the IFNgamma mimetic in antiviral therapies. STRUCTURED SUMMARY:

AB - Interferon (INF) gamma s ability to localise in the nucleus and function in gene activation has been known for some time, although the role of the conventional nuclear transporting importin molecules is unclear. Here, we demonstrate for the first time the direct recognition of IFNgamma and an IFNgamma mimetic peptide by IMPalpha and the IMPalpha/beta heterodimer, where the IFNgamma mimetic shows higher affinity. Significantly, this correlates well both with in vivo ability to target green fluorescent protein to the nucleus in transfected cells as determined by quantitative confocal laser scanning microscopy, as well as GAS promoter activity of a luciferase reporter. This has important implications for IFNgamma s anti-viral action, and the potential use of the IFNgamma mimetic in antiviral therapies. STRUCTURED SUMMARY:

U2 - 10.1016/j.febslet.2008.03.054

DO - 10.1016/j.febslet.2008.03.054

M3 - Article

SN - 0014-5793

VL - 582

SP - 1569

EP - 1574

JO - FEBS Letters

JF - FEBS Letters

IS - 11

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