Interface-specific x-ray phase retrieval tomography of complex biological organs

Mario Beltran, David Paganin, Karen Siu, Andreas Fouras, Stuart Hooper, David Reser, Marcus Kitchen

Research output: Contribution to journalArticleResearchpeer-review

Abstract

We demonstrate interface-specific propagation-based x-ray phase retrieval tomography of the thorax and brain of small animals. Our method utilizes a single propagation-based x-ray phase-contrast image per projection, under the assumptions of (i) partially coherent paraxial radiation, (ii) a static object whose refractive indices take on one of a series of distinct values at each point in space and (iii) the projection approximation. For the biological samples used here, there was a 9-200 fold improvement in the signal-to-noise ratio of the phase-retrieved tomograms over the conventional attenuation-contrast signal. The ability to digitally dissect a biological specimen, using only a single phase-contrast image per projection, will be useful for low-dose high-spatial-resolution biomedical imaging of form and biological function in both healthy and diseased tissue.
Original languageEnglish
Pages (from-to)7353 - 7369
Number of pages17
JournalPhysics in Medicine and Biology
Volume56
Issue number23
DOIs
Publication statusPublished - 2011

Cite this

Beltran, Mario ; Paganin, David ; Siu, Karen ; Fouras, Andreas ; Hooper, Stuart ; Reser, David ; Kitchen, Marcus. / Interface-specific x-ray phase retrieval tomography of complex biological organs. In: Physics in Medicine and Biology. 2011 ; Vol. 56, No. 23. pp. 7353 - 7369.
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Interface-specific x-ray phase retrieval tomography of complex biological organs. / Beltran, Mario; Paganin, David; Siu, Karen; Fouras, Andreas; Hooper, Stuart; Reser, David; Kitchen, Marcus.

In: Physics in Medicine and Biology, Vol. 56, No. 23, 2011, p. 7353 - 7369.

Research output: Contribution to journalArticleResearchpeer-review

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T1 - Interface-specific x-ray phase retrieval tomography of complex biological organs

AU - Beltran, Mario

AU - Paganin, David

AU - Siu, Karen

AU - Fouras, Andreas

AU - Hooper, Stuart

AU - Reser, David

AU - Kitchen, Marcus

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AB - We demonstrate interface-specific propagation-based x-ray phase retrieval tomography of the thorax and brain of small animals. Our method utilizes a single propagation-based x-ray phase-contrast image per projection, under the assumptions of (i) partially coherent paraxial radiation, (ii) a static object whose refractive indices take on one of a series of distinct values at each point in space and (iii) the projection approximation. For the biological samples used here, there was a 9-200 fold improvement in the signal-to-noise ratio of the phase-retrieved tomograms over the conventional attenuation-contrast signal. The ability to digitally dissect a biological specimen, using only a single phase-contrast image per projection, will be useful for low-dose high-spatial-resolution biomedical imaging of form and biological function in both healthy and diseased tissue.

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