Interaction of smoothened with integrin-linked kinase in primary cilia mediates Hedgehog signalling

Badia Maria Barakat, Liang Yu, Camden Yeung-Wah Lo, Duc Tri Vu, Elisabetta De Luca, Jason Cain, Luciano Gaston Martelotto, Shoukat Dedhar, Anthony John Sadler, Die Wang, David Neil Watkins, Gregory Hannigan

Research output: Contribution to journalArticleResearchpeer-review

19 Citations (Scopus)

Abstract

Here we report that ILK localizes in the mouse primary cilium, a sensory organelle required for signalling by the Hedgehog (Hh) pathway. Genetic or pharmacological inhibition of ILK blocks ciliary accumulation of the Hh pathway effector smoothened (Smo) and suppresses the induction of Gli transcription factor mRNAs by SHh. Conditional deletion of ILK or Smo also inhibits SHh-driven activation of Gli2 in the embryonic mouse cerebellum. ILK regulation of Hh signalling probably requires the physical interaction of ILK and Smo in the cilium, and we also show selective cilia-associated interaction of ILK with beta-arrestin, a known mediator of Smo-dependent signalling.
Original languageEnglish
Pages (from-to)837 - 944
Number of pages108
JournalEMBO Reports
Volume14
Issue number9
DOIs
Publication statusPublished - 2013

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