Interaction of Salmonella Typhimurium with Dendritic Cells Derived from Pluripotent Embryonic Stem Cells

Raffaella Rossi; Christine Hale; David A. Goulding; Robert Andrews; Zarah Abdellah; Paul J. Fairchild; Gordon Dougan

doi:10.1371/journal.pone.0052232

Interaction of Salmonella Typhimurium with Dendritic Cells Derived from Pluripotent Embryonic Stem Cells

Raffaella Rossi
, Christine Hale
, David A. Goulding
, Robert Andrews
, Zarah Abdellah
, Paul J. Fairchild
, Gordon Dougan

Research output: Contribution to journal › Article › Research › peer-review

8 Citations (Scopus)

Abstract

Using an in vitro differentiation protocol we isolated cells with the properties of dendritic cells (DCs) from immunologically refractive pluripotent murine embryonic stem cells (ESCs). These ES-derived dendritic cells (ESDCs) expressed cytokines and were able to present antigen to a T cell line. Infection of ESDCs with Salmonella Typhimurium stimulated the expression of immune cell markers and thousands of murine genes, many associated with the immune response. Consequently, this system provides a novel in vitro model, amenable to genetic modification, for monitoring host/pathogen interactions.

Original language	English
Article number	e52232
Journal	PLoS ONE
Volume	7
Issue number	12
DOIs	https://doi.org/10.1371/journal.pone.0052232
Publication status	Published - 28 Dec 2012
Externally published	Yes

Access to Document

10.1371/journal.pone.0052232

Cite this

@article{2bd69137b0ee4efaa0a87a1de5525130,

title = "Interaction of Salmonella Typhimurium with Dendritic Cells Derived from Pluripotent Embryonic Stem Cells",

abstract = "Using an in vitro differentiation protocol we isolated cells with the properties of dendritic cells (DCs) from immunologically refractive pluripotent murine embryonic stem cells (ESCs). These ES-derived dendritic cells (ESDCs) expressed cytokines and were able to present antigen to a T cell line. Infection of ESDCs with Salmonella Typhimurium stimulated the expression of immune cell markers and thousands of murine genes, many associated with the immune response. Consequently, this system provides a novel in vitro model, amenable to genetic modification, for monitoring host/pathogen interactions.",

author = "Raffaella Rossi and Christine Hale and Goulding, \{David A.\} and Robert Andrews and Zarah Abdellah and Fairchild, \{Paul J.\} and Gordon Dougan",

year = "2012",

month = dec,

day = "28",

doi = "10.1371/journal.pone.0052232",

language = "English",

volume = "7",

journal = "PLoS ONE",

issn = "1932-6203",

publisher = "Public Library of Science (PLoS)",

number = "12",

}

TY - JOUR

T1 - Interaction of Salmonella Typhimurium with Dendritic Cells Derived from Pluripotent Embryonic Stem Cells

AU - Rossi, Raffaella

AU - Hale, Christine

AU - Goulding, David A.

AU - Andrews, Robert

AU - Abdellah, Zarah

AU - Fairchild, Paul J.

AU - Dougan, Gordon

PY - 2012/12/28

Y1 - 2012/12/28

N2 - Using an in vitro differentiation protocol we isolated cells with the properties of dendritic cells (DCs) from immunologically refractive pluripotent murine embryonic stem cells (ESCs). These ES-derived dendritic cells (ESDCs) expressed cytokines and were able to present antigen to a T cell line. Infection of ESDCs with Salmonella Typhimurium stimulated the expression of immune cell markers and thousands of murine genes, many associated with the immune response. Consequently, this system provides a novel in vitro model, amenable to genetic modification, for monitoring host/pathogen interactions.

AB - Using an in vitro differentiation protocol we isolated cells with the properties of dendritic cells (DCs) from immunologically refractive pluripotent murine embryonic stem cells (ESCs). These ES-derived dendritic cells (ESDCs) expressed cytokines and were able to present antigen to a T cell line. Infection of ESDCs with Salmonella Typhimurium stimulated the expression of immune cell markers and thousands of murine genes, many associated with the immune response. Consequently, this system provides a novel in vitro model, amenable to genetic modification, for monitoring host/pathogen interactions.

UR - https://www.scopus.com/pages/publications/84871706051

U2 - 10.1371/journal.pone.0052232

DO - 10.1371/journal.pone.0052232

M3 - Article

C2 - 23284947

AN - SCOPUS:84871706051

SN - 1932-6203

VL - 7

JO - PLoS ONE

JF - PLoS ONE

IS - 12

M1 - e52232

ER -

Interaction of Salmonella Typhimurium with Dendritic Cells Derived from Pluripotent Embryonic Stem Cells

Abstract

Access to Document

Other files and links

Cite this