Interaction of Salmonella Typhimurium with Dendritic Cells Derived from Pluripotent Embryonic Stem Cells

Raffaella Rossi, Christine Hale, David A. Goulding, Robert Andrews, Zarah Abdellah, Paul J. Fairchild, Gordon Dougan

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Using an in vitro differentiation protocol we isolated cells with the properties of dendritic cells (DCs) from immunologically refractive pluripotent murine embryonic stem cells (ESCs). These ES-derived dendritic cells (ESDCs) expressed cytokines and were able to present antigen to a T cell line. Infection of ESDCs with Salmonella Typhimurium stimulated the expression of immune cell markers and thousands of murine genes, many associated with the immune response. Consequently, this system provides a novel in vitro model, amenable to genetic modification, for monitoring host/pathogen interactions.

Original languageEnglish
Article numbere52232
JournalPLoS ONE
Issue number12
Publication statusPublished - 28 Dec 2012
Externally publishedYes

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