Interaction of Salmonella Typhimurium with Dendritic Cells Derived from Pluripotent Embryonic Stem Cells

Raffaella Rossi; Christine Hale; David A. Goulding; Robert Andrews; Zarah Abdellah; Paul J. Fairchild; Gordon Dougan

doi:10.1371/journal.pone.0052232

Interaction of Salmonella Typhimurium with Dendritic Cells Derived from Pluripotent Embryonic Stem Cells

Raffaella Rossi, Christine Hale, David A. Goulding, Robert Andrews, Zarah Abdellah, Paul J. Fairchild, Gordon Dougan

Research output: Contribution to journal › Article › Research › peer-review

7 Citations (Scopus)

Abstract

Using an in vitro differentiation protocol we isolated cells with the properties of dendritic cells (DCs) from immunologically refractive pluripotent murine embryonic stem cells (ESCs). These ES-derived dendritic cells (ESDCs) expressed cytokines and were able to present antigen to a T cell line. Infection of ESDCs with Salmonella Typhimurium stimulated the expression of immune cell markers and thousands of murine genes, many associated with the immune response. Consequently, this system provides a novel in vitro model, amenable to genetic modification, for monitoring host/pathogen interactions.

Original language	English
Article number	e52232
Journal	PLoS ONE
Volume	7
Issue number	12
DOIs	https://doi.org/10.1371/journal.pone.0052232
Publication status	Published - 28 Dec 2012
Externally published	Yes

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10.1371/journal.pone.0052232

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Cite this

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abstract = "Using an in vitro differentiation protocol we isolated cells with the properties of dendritic cells (DCs) from immunologically refractive pluripotent murine embryonic stem cells (ESCs). These ES-derived dendritic cells (ESDCs) expressed cytokines and were able to present antigen to a T cell line. Infection of ESDCs with Salmonella Typhimurium stimulated the expression of immune cell markers and thousands of murine genes, many associated with the immune response. Consequently, this system provides a novel in vitro model, amenable to genetic modification, for monitoring host/pathogen interactions.",

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AU - Goulding, David A.

AU - Andrews, Robert

AU - Abdellah, Zarah

AU - Fairchild, Paul J.

AU - Dougan, Gordon

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