Interaction of merozoite surface protein 2 with lipid membranes

Chenghui Lu, Xue Zheng, Wei Zhang, Hongxin Zhao, Christopher A. MacRaild, Raymond S. Norton, Yonglong Zhuang, Junfeng Wang, Xuecheng Zhang

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Merozoite surface protein 2 (MSP2) is a potential vaccine candidate against malaria, although its functional role is yet to be elucidated. Previous studies showed that MSP2 can interact with membranes, which may facilitate merozoite invasion into the host cell. The N-terminal 25 residues of MSP2 (MSP21–25), which may be aggregated on the merozoite surface, play a key role in the interaction with membranes. Here, we investigated the effects of MSP21–25–membrane interactions on the conformation and aggregation of MSP21–25 and on membrane integrity, using nanodiscs and small unilamellar vesicles as mimetics of cell membranes. MSP21–25–membrane interactions induced the peptide to form β-structure and to aggregate, depending on the lipid composition of the membrane. Nonfibrillar aggregates in turn disrupted the membrane. (Figure presented.).

Original languageEnglish
Pages (from-to)288-295
Number of pages8
JournalFEBS Letters
Volume593
Issue number3
DOIs
Publication statusPublished - Feb 2019

Keywords

  • aggregation
  • amyloid fibril
  • membrane disruption
  • membrane interaction
  • merozoite surface protein 2

Cite this

Lu, Chenghui ; Zheng, Xue ; Zhang, Wei ; Zhao, Hongxin ; MacRaild, Christopher A. ; Norton, Raymond S. ; Zhuang, Yonglong ; Wang, Junfeng ; Zhang, Xuecheng. / Interaction of merozoite surface protein 2 with lipid membranes. In: FEBS Letters. 2019 ; Vol. 593, No. 3. pp. 288-295.
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title = "Interaction of merozoite surface protein 2 with lipid membranes",
abstract = "Merozoite surface protein 2 (MSP2) is a potential vaccine candidate against malaria, although its functional role is yet to be elucidated. Previous studies showed that MSP2 can interact with membranes, which may facilitate merozoite invasion into the host cell. The N-terminal 25 residues of MSP2 (MSP21–25), which may be aggregated on the merozoite surface, play a key role in the interaction with membranes. Here, we investigated the effects of MSP21–25–membrane interactions on the conformation and aggregation of MSP21–25 and on membrane integrity, using nanodiscs and small unilamellar vesicles as mimetics of cell membranes. MSP21–25–membrane interactions induced the peptide to form β-structure and to aggregate, depending on the lipid composition of the membrane. Nonfibrillar aggregates in turn disrupted the membrane. (Figure presented.).",
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author = "Chenghui Lu and Xue Zheng and Wei Zhang and Hongxin Zhao and MacRaild, {Christopher A.} and Norton, {Raymond S.} and Yonglong Zhuang and Junfeng Wang and Xuecheng Zhang",
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Lu, C, Zheng, X, Zhang, W, Zhao, H, MacRaild, CA, Norton, RS, Zhuang, Y, Wang, J & Zhang, X 2019, 'Interaction of merozoite surface protein 2 with lipid membranes' FEBS Letters, vol. 593, no. 3, pp. 288-295. https://doi.org/10.1002/1873-3468.13320

Interaction of merozoite surface protein 2 with lipid membranes. / Lu, Chenghui; Zheng, Xue; Zhang, Wei; Zhao, Hongxin; MacRaild, Christopher A.; Norton, Raymond S.; Zhuang, Yonglong; Wang, Junfeng; Zhang, Xuecheng.

In: FEBS Letters, Vol. 593, No. 3, 02.2019, p. 288-295.

Research output: Contribution to journalArticleResearchpeer-review

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AU - Lu, Chenghui

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