Interaction of annexin V and platelets

Effects on platelet function and protein S binding

Research output: Contribution to journalArticleResearchpeer-review

16 Citations (Scopus)

Abstract

Intact annexin V but not its fragments bound specifically to platelets in the presence of calcium. Maximal binding observed was 0.87 pmole annexin V per 108 platelets with an apparent dissociation constant of 2.13 × 1011 M. This represents approximately 5000 binding sites per platelet. Platelet stimulation by thrombin increased the binding of annexin V by 40-fold. Addition of phospholipid vesicles or treatment with phospholipase C inhibited annexin V binding to platelets, suggesting that phospholipid is the binding site for annexin V and that phosphatidylcholine forms at least part of this site. Binding of annexin V did not cause platelet aggregation and did not affect thrombin or collagen induced aggregation. However annexin V significantly inhibited the binding of protein S to the platelet surface.

Original languageEnglish
Pages (from-to)289-296
Number of pages8
JournalThrombosis Research
Volume69
Issue number3
DOIs
Publication statusPublished - 1 Feb 1993
Externally publishedYes

Keywords

  • annexin V
  • phosphatidylcholine
  • phospholipid
  • Platelets
  • protein S

Cite this

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abstract = "Intact annexin V but not its fragments bound specifically to platelets in the presence of calcium. Maximal binding observed was 0.87 pmole annexin V per 108 platelets with an apparent dissociation constant of 2.13 × 1011 M. This represents approximately 5000 binding sites per platelet. Platelet stimulation by thrombin increased the binding of annexin V by 40-fold. Addition of phospholipid vesicles or treatment with phospholipase C inhibited annexin V binding to platelets, suggesting that phospholipid is the binding site for annexin V and that phosphatidylcholine forms at least part of this site. Binding of annexin V did not cause platelet aggregation and did not affect thrombin or collagen induced aggregation. However annexin V significantly inhibited the binding of protein S to the platelet surface.",
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Interaction of annexin V and platelets : Effects on platelet function and protein S binding. / Sun, Jiuru; Bird, Phillip; Salem, Hatem H.

In: Thrombosis Research, Vol. 69, No. 3, 01.02.1993, p. 289-296.

Research output: Contribution to journalArticleResearchpeer-review

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