The granular starch binding domain of glucoamyla 1 (EC 126.96.36.199 1,4-α-d-glucan glucohydrolase) binds two molecules of β-cyclodextrin, with a dissociation constant (Kd) for the second ligand of 1.68 μM. The catalytic domain showed no interaction with β-cyclodextrin. β-cyclodextrin competitively inhibited the adsorption of the binding domain onto granular starch with an inhibition constant (Ki) of 11.0 ± 1.9 μM. The results show that β-cyclodextrin binds to the binding domain of glucoamylase at the same site(s) as granular starch.
|Number of pages||4|
|Journal||Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology|
|Publication status||Published - 30 May 1991|
- Binding domain