Integration of Hedgehog and mutant FLT3 signaling in myeloid leukemia

Yiting Lim, Lukasz Gondek, Li Li, Qiuju Wang, Haley Ma, Emily Chang, David L. Huso, Sarah Foerster, Luigi Marchionni, Karen McGovern, David Neil Watkins, Craig D. Peacock, Mark Levis, Bruce Douglas Smith, Akil A. Merchant, Donald Small, William Matsui

Research output: Contribution to journalArticleResearchpeer-review

25 Citations (Scopus)

Abstract

FMS-like tyrosine kinase 3 (FLT3) internal tandem duplication (ITD) mutations resulting in constitutive kinase activity are common in acute myeloid leukemia (AML) and carry a poor prognosis. Several agents targeting FLT3 have been developed, but their limited clinical activity suggests that the inhibition of other factors contributing to the malignant phenotype is required. We examined gene expression data sets as well as primary specimens and found that the expression of GLI2, a major effector of the Hedgehog (Hh) signaling pathway, was increased in FLT3-ITD compared to wild-type FLT3 AML. To examine the functional role of the Hh pathway, we studied mice in which Flt3-ITD expression results in an indolent myeloproliferative state and found that constitutive Hh signaling accelerated the development of AML by enhancing signal transducer and activator of transcription 5 (STAT5) signaling and the proliferation of bone marrow myeloid progenitors. Furthermore, combined FLT3 and Hh pathway inhibition limited leukemic growth in vitro and in vivo, and this approach may serve as a therapeutic strategy for FLT3-ITD AML.

Original languageEnglish
Article number291ra96
Number of pages12
JournalScience Translational Medicine
Volume7
Issue number291
DOIs
Publication statusPublished - 10 Jun 2015
Externally publishedYes

Cite this