TY - JOUR
T1 - Integrating piggyBac transposon transgenes into mouse fibroblasts by electroporation
AU - Behringer, Richard
AU - Gertsenstein, Marina
AU - Vintersten Nagy, Kristina
AU - Nagy, Andras
PY - 2017/10/1
Y1 - 2017/10/1
N2 - Mouse embryonic fibroblasts can be reprogrammed to embryonic stem (ES) cell-like pluripotent stem cells by the forced expression of four transcription factors—OCT4, SOX2, KLF4, and c-MYC. The piggyBac transposon system has proven effective as a vehicle for the delivery of transgenes into fibroblasts and for successful reprogramming to induced pluripotent stem (iPS) cells. This protocol is designed for use with the Neon electroporation system. It can be adapted to other types of electroporation systems.
AB - Mouse embryonic fibroblasts can be reprogrammed to embryonic stem (ES) cell-like pluripotent stem cells by the forced expression of four transcription factors—OCT4, SOX2, KLF4, and c-MYC. The piggyBac transposon system has proven effective as a vehicle for the delivery of transgenes into fibroblasts and for successful reprogramming to induced pluripotent stem (iPS) cells. This protocol is designed for use with the Neon electroporation system. It can be adapted to other types of electroporation systems.
UR - http://www.scopus.com/inward/record.url?scp=85030632440&partnerID=8YFLogxK
U2 - 10.1101/pdb.prot092601
DO - 10.1101/pdb.prot092601
M3 - Article
C2 - 28974651
AN - SCOPUS:85030632440
SN - 1940-3402
VL - 2017
SP - 831
EP - 833
JO - Cold Spring Harbor Protocols
JF - Cold Spring Harbor Protocols
IS - 10
ER -