Integrating piggyBac transposon transgenes into mouse fibroblasts by electroporation

Richard Behringer, Marina Gertsenstein, Kristina Vintersten Nagy, Andras Nagy

Research output: Contribution to journalArticleOtherpeer-review

2 Citations (Scopus)


Mouse embryonic fibroblasts can be reprogrammed to embryonic stem (ES) cell-like pluripotent stem cells by the forced expression of four transcription factors—OCT4, SOX2, KLF4, and c-MYC. The piggyBac transposon system has proven effective as a vehicle for the delivery of transgenes into fibroblasts and for successful reprogramming to induced pluripotent stem (iPS) cells. This protocol is designed for use with the Neon electroporation system. It can be adapted to other types of electroporation systems.

Original languageEnglish
Pages (from-to)831-833
Number of pages3
JournalCold Spring Harbor Protocols
Issue number10
Publication statusPublished - 1 Oct 2017

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