Integrating piggyBac transposon transgenes into mouse fibroblasts by electroporation

Richard Behringer, Marina Gertsenstein, Kristina Vintersten Nagy, Andras Nagy

Research output: Contribution to journalArticleOtherpeer-review

Abstract

Mouse embryonic fibroblasts can be reprogrammed to embryonic stem (ES) cell-like pluripotent stem cells by the forced expression of four transcription factors—OCT4, SOX2, KLF4, and c-MYC. The piggyBac transposon system has proven effective as a vehicle for the delivery of transgenes into fibroblasts and for successful reprogramming to induced pluripotent stem (iPS) cells. This protocol is designed for use with the Neon electroporation system. It can be adapted to other types of electroporation systems.

Original languageEnglish
Pages (from-to)831-833
Number of pages3
JournalCold Spring Harbor Protocols
Volume2017
Issue number10
DOIs
Publication statusPublished - 1 Oct 2017

Cite this

Behringer, Richard ; Gertsenstein, Marina ; Vintersten Nagy, Kristina ; Nagy, Andras. / Integrating piggyBac transposon transgenes into mouse fibroblasts by electroporation. In: Cold Spring Harbor Protocols. 2017 ; Vol. 2017, No. 10. pp. 831-833.
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Integrating piggyBac transposon transgenes into mouse fibroblasts by electroporation. / Behringer, Richard; Gertsenstein, Marina; Vintersten Nagy, Kristina; Nagy, Andras.

In: Cold Spring Harbor Protocols, Vol. 2017, No. 10, 01.10.2017, p. 831-833.

Research output: Contribution to journalArticleOtherpeer-review

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