Integrating piggyBac transposon transgenes into mouse fibroblasts using chemical methods

Richard Behringer, Marina Gertsenstein, Kristina Vintersten Nagy, Andras Nagy

Research output: Contribution to journalArticleOtherpeer-review

2 Citations (Scopus)

Abstract

Mouse embryonic fibroblasts can be reprogrammed to ES cell-like pluripotent stem cells by the forced expression of four transcription factors—OCT4, SOX2, KLF4, and c-MYC. The piggyBac transposon system has proven effective as a vehicle for the delivery of transgenes into fibroblasts and for successful reprogramming to induced pluripotent stem (iPS) cells. We found that FuGENE HD transfection reagent can be effective for mouse embryonic fibroblasts (MEFs) to generate induced pluripotent stem cells (iPSCs) with the piggyBac transposon transgenes. There are multitudes of cell transfection methods commercially available. Their efficiency, and thus their success, in inducing pluripotent cell types are cell-type-dependent.

Original languageEnglish
Pages (from-to)834-836
Number of pages3
JournalCold Spring Harbor Protocols
Volume2017
Issue number10
DOIs
Publication statusPublished - 1 Oct 2017

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