TY - JOUR
T1 - Integrating piggyBac transposon transgenes into mouse fibroblasts using chemical methods
AU - Behringer, Richard
AU - Gertsenstein, Marina
AU - Vintersten Nagy, Kristina
AU - Nagy, Andras
PY - 2017/10/1
Y1 - 2017/10/1
N2 - Mouse embryonic fibroblasts can be reprogrammed to ES cell-like pluripotent stem cells by the forced expression of four transcription factors—OCT4, SOX2, KLF4, and c-MYC. The piggyBac transposon system has proven effective as a vehicle for the delivery of transgenes into fibroblasts and for successful reprogramming to induced pluripotent stem (iPS) cells. We found that FuGENE HD transfection reagent can be effective for mouse embryonic fibroblasts (MEFs) to generate induced pluripotent stem cells (iPSCs) with the piggyBac transposon transgenes. There are multitudes of cell transfection methods commercially available. Their efficiency, and thus their success, in inducing pluripotent cell types are cell-type-dependent.
AB - Mouse embryonic fibroblasts can be reprogrammed to ES cell-like pluripotent stem cells by the forced expression of four transcription factors—OCT4, SOX2, KLF4, and c-MYC. The piggyBac transposon system has proven effective as a vehicle for the delivery of transgenes into fibroblasts and for successful reprogramming to induced pluripotent stem (iPS) cells. We found that FuGENE HD transfection reagent can be effective for mouse embryonic fibroblasts (MEFs) to generate induced pluripotent stem cells (iPSCs) with the piggyBac transposon transgenes. There are multitudes of cell transfection methods commercially available. Their efficiency, and thus their success, in inducing pluripotent cell types are cell-type-dependent.
UR - http://www.scopus.com/inward/record.url?scp=85030638816&partnerID=8YFLogxK
U2 - 10.1101/pdb.prot092619
DO - 10.1101/pdb.prot092619
M3 - Article
C2 - 28974652
AN - SCOPUS:85030638816
SN - 1940-3402
VL - 2017
SP - 834
EP - 836
JO - Cold Spring Harbor Protocols
JF - Cold Spring Harbor Protocols
IS - 10
ER -