TY - JOUR
T1 - Instrumental biosensors
T2 - New perspectives for the analysis of biomolecular interactions
AU - Nice, E. C.
AU - Catimel, B.
PY - 1999/4/1
Y1 - 1999/4/1
N2 - The use of instrumental biosensors in basic research to measure biomolecular interactions in real time is increasing exponentially. Applications include protein-protein, protein-peptide, DNA-protein, DNA-DNA, and lipid-protein interactions. Such techniques have been applied to, for example, antibody-antigen, receptor-ligand, signal transduction, and nuclear receptor studies. This review outlines the principles of two of the most commonly used instruments and highlights specific operating parameters that will assist in optimising experimental design, data generation, and analysis.
AB - The use of instrumental biosensors in basic research to measure biomolecular interactions in real time is increasing exponentially. Applications include protein-protein, protein-peptide, DNA-protein, DNA-DNA, and lipid-protein interactions. Such techniques have been applied to, for example, antibody-antigen, receptor-ligand, signal transduction, and nuclear receptor studies. This review outlines the principles of two of the most commonly used instruments and highlights specific operating parameters that will assist in optimising experimental design, data generation, and analysis.
UR - http://www.scopus.com/inward/record.url?scp=0033119556&partnerID=8YFLogxK
U2 - 10.1002/(SICI)1521-1878(199904)21:4<339::AID-BIES11>3.0.CO;2-C
DO - 10.1002/(SICI)1521-1878(199904)21:4<339::AID-BIES11>3.0.CO;2-C
M3 - Article
C2 - 10377896
AN - SCOPUS:0033119556
VL - 21
SP - 339
EP - 352
JO - BioEssays
JF - BioEssays
SN - 0265-9247
IS - 4
ER -