Instrumental biosensors: New perspectives for the analysis of biomolecular interactions

E. C. Nice, B. Catimel

Research output: Contribution to journalArticleResearchpeer-review

182 Citations (Scopus)

Abstract

The use of instrumental biosensors in basic research to measure biomolecular interactions in real time is increasing exponentially. Applications include protein-protein, protein-peptide, DNA-protein, DNA-DNA, and lipid-protein interactions. Such techniques have been applied to, for example, antibody-antigen, receptor-ligand, signal transduction, and nuclear receptor studies. This review outlines the principles of two of the most commonly used instruments and highlights specific operating parameters that will assist in optimising experimental design, data generation, and analysis.

Original languageEnglish
Pages (from-to)339-352
Number of pages14
JournalBioEssays
Volume21
Issue number4
DOIs
Publication statusPublished - 1 Apr 1999
Externally publishedYes

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