INSL5 activates multiple signalling pathways and regulates GLP-1 secretion in NCI-H716 cells

Sheng Y. Ang, Bronwyn A. Evans, Daniel P. Poole, Romke Bron, Jesse J. DiCello, Ross A.D. Bathgate, Martina Kocan, Dana S. Hutchinson, Roger J. Summers

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Insulin-like peptide 5 (INSL5) is a newly discovered gut hormone expressed in colonic enteroendocrine L-cells but little is known about its biological function. Here, we show using RT-qPCR and in situ hybridisation that Insl5 mRNA is highly expressed in the mouse colonic mucosa, colocalised with proglucagon immunoreactivity. In comparison, mRNA for RXFP4 (the cognate receptor for INSL5) is expressed in various mouse tissues, including the intestinal tract. We show that the human enteroendocrine L-cell model NCI-H716 cell line, and goblet-like colorectal cell lines SW1463 and LS513 endogenously express RXFP4. Stimulation of NCI-H716 cells with INSL5 produced phosphorylation of ERK1/2 (Thr202/Tyr204), AKT (Thr308 and Ser473) and S6RP (Ser235/236) and inhibited cAMP production but did not stimulate Ca2+ release. Acute INSL5 treatment had no effect on GLP-1 secretion mediated by carbachol or insulin, but modestly inhibited forskolin-stimulated GLP-1 secretion in NCI-H716 cells. However, chronic INSL5 pre-treatment (18 h) increased basal GLP-1 secretion and prevented the inhibitory effect of acute INSL5 administration. LS513 cells were found to be unresponsive to INSL5 despite expressing RXFP4. Another enteroendocrine L-cell model, mouse GLUTag cells did not express detectable levels of Rxfp4 and were unresponsive to INSL5. This study provides novel insights into possible autocrine/paracrine roles of INSL5 in the intestinal tract.

Original languageEnglish
Pages (from-to)213-224
Number of pages12
JournalJournal of Molecular Endocrinology
Volume60
Issue number3
DOIs
Publication statusPublished - 1 Apr 2018

Keywords

  • Cell signaling
  • GLP-1
  • INSL5
  • NCI-H716
  • RXFP4

Cite this

Ang, Sheng Y. ; Evans, Bronwyn A. ; Poole, Daniel P. ; Bron, Romke ; DiCello, Jesse J. ; Bathgate, Ross A.D. ; Kocan, Martina ; Hutchinson, Dana S. ; Summers, Roger J. / INSL5 activates multiple signalling pathways and regulates GLP-1 secretion in NCI-H716 cells. In: Journal of Molecular Endocrinology. 2018 ; Vol. 60, No. 3. pp. 213-224.
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abstract = "Insulin-like peptide 5 (INSL5) is a newly discovered gut hormone expressed in colonic enteroendocrine L-cells but little is known about its biological function. Here, we show using RT-qPCR and in situ hybridisation that Insl5 mRNA is highly expressed in the mouse colonic mucosa, colocalised with proglucagon immunoreactivity. In comparison, mRNA for RXFP4 (the cognate receptor for INSL5) is expressed in various mouse tissues, including the intestinal tract. We show that the human enteroendocrine L-cell model NCI-H716 cell line, and goblet-like colorectal cell lines SW1463 and LS513 endogenously express RXFP4. Stimulation of NCI-H716 cells with INSL5 produced phosphorylation of ERK1/2 (Thr202/Tyr204), AKT (Thr308 and Ser473) and S6RP (Ser235/236) and inhibited cAMP production but did not stimulate Ca2+ release. Acute INSL5 treatment had no effect on GLP-1 secretion mediated by carbachol or insulin, but modestly inhibited forskolin-stimulated GLP-1 secretion in NCI-H716 cells. However, chronic INSL5 pre-treatment (18 h) increased basal GLP-1 secretion and prevented the inhibitory effect of acute INSL5 administration. LS513 cells were found to be unresponsive to INSL5 despite expressing RXFP4. Another enteroendocrine L-cell model, mouse GLUTag cells did not express detectable levels of Rxfp4 and were unresponsive to INSL5. This study provides novel insights into possible autocrine/paracrine roles of INSL5 in the intestinal tract.",
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INSL5 activates multiple signalling pathways and regulates GLP-1 secretion in NCI-H716 cells. / Ang, Sheng Y.; Evans, Bronwyn A.; Poole, Daniel P.; Bron, Romke; DiCello, Jesse J.; Bathgate, Ross A.D.; Kocan, Martina; Hutchinson, Dana S.; Summers, Roger J.

In: Journal of Molecular Endocrinology, Vol. 60, No. 3, 01.04.2018, p. 213-224.

Research output: Contribution to journalArticleResearchpeer-review

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AU - Evans, Bronwyn A.

AU - Poole, Daniel P.

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AU - DiCello, Jesse J.

AU - Bathgate, Ross A.D.

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