TY - JOUR
T1 - Insights into the organization of dorsal spinal cord pathways from an evolutionarily conserved raldh2 intronic enhancer
AU - Castillo, Hozana A
AU - Cravo, Roberta M
AU - Azambuja, Ana P
AU - Simoes-Costa, Marcos S
AU - Sura-Trueba, Sylvia
AU - Gonzalez, Jose
AU - Slonimsky, Esfir
AU - Almeida, Karla
AU - Abreu, Jose G
AU - de Almeida, Marcio A Alfonso
AU - Sobreira, Tiago P
AU - Pires de Oliveira, Saulo H
AU - de Oliveira, Paulo S Lopes
AU - Signore, Iskra A
AU - Colombo, Alicia
AU - Concha, Miguel L
AU - Spengler, Tatjana S
AU - Bronner-Fraser, Marianne
AU - Nobrega, Marcelo
AU - Rosenthal, Nadia
AU - Xavier-Neto, Jose
PY - 2010
Y1 - 2010
N2 - Comparative studies of the tetrapod raldh2 (aldh1a2) gene, which encodes a retinoic acid (RA) synthesis enzyme, have led to the identification of a dorsal spinal cord enhancer. Enhancer activity is directed dorsally to the roof plate and dorsal-most (dI1) interneurons through predicted Tcf- and Cdx-homeodomain binding sites and is repressed ventrally via predicted Tgif homeobox and ventral Lim-homeodomain binding sites. Raldh2 and Math1/Cath1 expression in mouse and chicken highlights a novel, transient, endogenous Raldh2 expression domain in dI1 interneurons, which give rise to ascending circuits and intraspinal commissural interneurons, suggesting roles for RA in the ontogeny of spinocerebellar and intraspinal proprioceptive circuits. Consistent with expression of raldh2 in the dorsal interneurons of tetrapods, we also found that raldh2 is expressed in dorsal interneurons throughout the agnathan spinal cord, suggesting ancestral roles for RA signaling in the ontogenesis of intraspinal proprioception.
AB - Comparative studies of the tetrapod raldh2 (aldh1a2) gene, which encodes a retinoic acid (RA) synthesis enzyme, have led to the identification of a dorsal spinal cord enhancer. Enhancer activity is directed dorsally to the roof plate and dorsal-most (dI1) interneurons through predicted Tcf- and Cdx-homeodomain binding sites and is repressed ventrally via predicted Tgif homeobox and ventral Lim-homeodomain binding sites. Raldh2 and Math1/Cath1 expression in mouse and chicken highlights a novel, transient, endogenous Raldh2 expression domain in dI1 interneurons, which give rise to ascending circuits and intraspinal commissural interneurons, suggesting roles for RA in the ontogeny of spinocerebellar and intraspinal proprioceptive circuits. Consistent with expression of raldh2 in the dorsal interneurons of tetrapods, we also found that raldh2 is expressed in dorsal interneurons throughout the agnathan spinal cord, suggesting ancestral roles for RA signaling in the ontogenesis of intraspinal proprioception.
UR - http://dev.biologists.org/content/137/3/507.full.pdf+html
U2 - 10.1242/dev.043257
DO - 10.1242/dev.043257
M3 - Article
SN - 0950-1991
VL - 137
SP - 507
EP - 518
JO - Development
JF - Development
IS - 3
ER -