Insight in the quorum sensing-driven lifestyle of the non-pathogenic Agrobacterium tumefaciens 6N2 and the interactions with the yeast Meyerozyma guilliermondii

Elisa Violeta Bertini, Mariela Analía Torres, Thibaut Léger, Camille Garcia, Kar Wai Hong, Teik Min Chong, Lucía I. Castellanos de Figueroa, Kok Gan Chan, Yves Dessaux, Jean Michel Camadro, Carlos Gabriel Nieto-Peñalver

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Agrobacterium tumefaciens is considered a prominent phytopathogen, though most isolates are nonpathogenic. Agrobacteria can inhabit plant tissues interacting with other microorganisms. Yeasts are likewise part of these communities. We analyzed the quorum sensing (QS) systems of A. tumefaciens strain 6N2, and its relevance for the interaction with the yeast Meyerozyma guilliermondii, both sugarcane endophytes. We show that strain 6N2 is nonpathogenic, produces OHC8-HSL, OHC10-HSL, OC12-HSL and OHC12-HSL as QS signals, and possesses a complex QS architecture, with one truncated, two complete systems, and three additional QS-signal receptors. A proteomic approach showed differences in QS-regulated proteins between pure (64 proteins) and dual (33 proteins) cultures. Seven proteins were consistently regulated by quorum sensing in pure and dual cultures. M. guilliermondii proteins influenced by QS activity were also evaluated. Several up- and down- regulated proteins differed depending on the bacterial QS. These results show the QS regulation in the bacteria-yeast interactions.

Original languageEnglish
Pages (from-to)4352-4360
Number of pages9
JournalGenomics
Volume113
Issue number6
DOIs
Publication statusPublished - Nov 2021
Externally publishedYes

Keywords

  • Agrobacteria
  • Endophytic
  • Interactions
  • Proteomic
  • Quorum sensing

Cite this