Inhibition of release of inflammatory mediators in rat peritoneal mast cells and murine macrophages by a chinese herbal medicine formula (RCM-102)

George Binh Lenon, Charlie Changli Xue, David F Story, Francis Thien, Chun Guang Li

Research output: Contribution to journalArticleResearchpeer-review

1 Citation (Scopus)

Abstract

RCM-102 is a Chinese herbal medicine formulation derived from a formula which was shown to be effective in treating seasonal allergic rhinitis (SAR) in a randomized placebo-controlled trial. The aim of this study was to investigate the in vitro effect of RCM-102 on the formation of inflammatory mediators, histamine, prostaglandin and nitric oxide, which are known to be involved in the pathophysiology of SAR. The effect of RCM-102 on histamine release was tested in compound 48/80-stimulated rat peritoneal mast cells. The effects of RCM-102 on the release of NO and prostaglandins (PGE2) and the expression of inducible NO synthase (iNOS) and COX-2 were studied in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells. In rat peritoneal mast cells, RCM-102 significantly reduced the compound 48/80-induced histamine release. It also significantly reduced NO and PGE2 production as well as the expression of COX-2 and iNOS in RAW 264.7 cells. These findings indicate that RCM-102 inhibits the formation of several allergic/inflammatory mediators and thus may be used for treating related conditions such as SAR. The actions of RCM-102 are likely to be contributed by the synergistic actions of individual herbal ingredients
Original languageEnglish
Pages (from-to)1270 - 1275
Number of pages6
JournalPhytotherapy Research
Volume23
Issue number9
DOIs
Publication statusPublished - 2009

Cite this