Inhibition of lysosomal vacuolar proton pump down-regulates cellular acidification and enhances E. coli bactofection efficiency

Rasaq Olajide Akinsola; Choon Weng Lee; Edmund Ui Hang Sim; Kumaran Narayanan

doi:10.1016/j.ab.2020.114088

Inhibition of lysosomal vacuolar proton pump down-regulates cellular acidification and enhances E. coli bactofection efficiency

Rasaq Olajide Akinsola
, Choon Weng Lee
, Edmund Ui Hang Sim
, Kumaran Narayanan

Malaysia Sch of Science

Research output: Contribution to journal › Article › Other › peer-review

2 Citations (Scopus)

Abstract

Endosomal escape is considered a crucial barrier that needs to be overcome by integrin-mediated E. coli for gene delivery into mammalian cells. Bafilomycin, a potent inhibitor of the H+ proton pump commonly employed to lower endosomal pH, was evaluated as part of the E. coli protocol during delivery. We found an increase in green fluorescent protein expression up 6.9, 3.2, 5.0, 2.8, and 4.5 fold in HeLa, HEK-293, A549, HT1080, and MCF-7 respectively, compared to untreated cells. Our result showed for the first time that Inhibition of lysosomal V-ATPase enhances E. coli efficiency.

Original language	English
Article number	114088
Number of pages	3
Journal	Analytical Biochemistry
Volume	616
DOIs	https://doi.org/10.1016/j.ab.2020.114088
Publication status	Published - 1 Mar 2021

Keywords

Bacteria DNA delivery
Endocytosed
Endosome
Lysosome
Proton pump inhibitor
V-ATPase

Access to Document

10.1016/j.ab.2020.114088

Cite this

@article{3c018ab616ec4598b14df51fad359ce8,

title = "Inhibition of lysosomal vacuolar proton pump down-regulates cellular acidification and enhances E. coli bactofection efficiency",

abstract = "Endosomal escape is considered a crucial barrier that needs to be overcome by integrin-mediated E. coli for gene delivery into mammalian cells. Bafilomycin, a potent inhibitor of the H+ proton pump commonly employed to lower endosomal pH, was evaluated as part of the E. coli protocol during delivery. We found an increase in green fluorescent protein expression up 6.9, 3.2, 5.0, 2.8, and 4.5 fold in HeLa, HEK-293, A549, HT1080, and MCF-7 respectively, compared to untreated cells. Our result showed for the first time that Inhibition of lysosomal V-ATPase enhances E. coli efficiency.",

keywords = "Bacteria DNA delivery, Endocytosed, Endosome, Lysosome, Proton pump inhibitor, V-ATPase",

author = "Akinsola, \{Rasaq Olajide\} and Lee, \{Choon Weng\} and Sim, \{Edmund Ui Hang\} and Kumaran Narayanan",

note = "Funding Information: This work was funded by a MOSTI eScience grant 02-02-10-SF0252 from the Ministry of Science, Technology, and Innovation, Malaysia, to KN. RA is a PhD student supported by a Higher Degree by Research (HDR) Scholarship from Monash University Malaysia. Publisher Copyright: {\textcopyright} 2020 Elsevier Inc. Copyright: Copyright 2021 Elsevier B.V., All rights reserved.",

year = "2021",

month = mar,

day = "1",

doi = "10.1016/j.ab.2020.114088",

language = "English",

volume = "616",

journal = "Analytical Biochemistry",

issn = "0003-2697",

publisher = "Elsevier",

}

TY - JOUR

T1 - Inhibition of lysosomal vacuolar proton pump down-regulates cellular acidification and enhances E. coli bactofection efficiency

AU - Akinsola, Rasaq Olajide

AU - Lee, Choon Weng

AU - Sim, Edmund Ui Hang

AU - Narayanan, Kumaran

N1 - Funding Information: This work was funded by a MOSTI eScience grant 02-02-10-SF0252 from the Ministry of Science, Technology, and Innovation, Malaysia, to KN. RA is a PhD student supported by a Higher Degree by Research (HDR) Scholarship from Monash University Malaysia. Publisher Copyright: © 2020 Elsevier Inc. Copyright: Copyright 2021 Elsevier B.V., All rights reserved.

PY - 2021/3/1

Y1 - 2021/3/1

N2 - Endosomal escape is considered a crucial barrier that needs to be overcome by integrin-mediated E. coli for gene delivery into mammalian cells. Bafilomycin, a potent inhibitor of the H+ proton pump commonly employed to lower endosomal pH, was evaluated as part of the E. coli protocol during delivery. We found an increase in green fluorescent protein expression up 6.9, 3.2, 5.0, 2.8, and 4.5 fold in HeLa, HEK-293, A549, HT1080, and MCF-7 respectively, compared to untreated cells. Our result showed for the first time that Inhibition of lysosomal V-ATPase enhances E. coli efficiency.

AB - Endosomal escape is considered a crucial barrier that needs to be overcome by integrin-mediated E. coli for gene delivery into mammalian cells. Bafilomycin, a potent inhibitor of the H+ proton pump commonly employed to lower endosomal pH, was evaluated as part of the E. coli protocol during delivery. We found an increase in green fluorescent protein expression up 6.9, 3.2, 5.0, 2.8, and 4.5 fold in HeLa, HEK-293, A549, HT1080, and MCF-7 respectively, compared to untreated cells. Our result showed for the first time that Inhibition of lysosomal V-ATPase enhances E. coli efficiency.

KW - Bacteria DNA delivery

KW - Endocytosed

KW - Endosome

KW - Lysosome

KW - Proton pump inhibitor

KW - V-ATPase

UR - https://www.scopus.com/pages/publications/85098562577

U2 - 10.1016/j.ab.2020.114088

DO - 10.1016/j.ab.2020.114088

M3 - Article

C2 - 33358938

AN - SCOPUS:85098562577

SN - 0003-2697

VL - 616

JO - Analytical Biochemistry

JF - Analytical Biochemistry

M1 - 114088

ER -

Inhibition of lysosomal vacuolar proton pump down-regulates cellular acidification and enhances E. coli bactofection efficiency

Abstract

Keywords

Access to Document

Other files and links

Advancing E.coli as a Vector for Gene Delivery into Human Cells: Hybird with DNA /protein delivery reagents and intracellular strategies

Cite this

Inhibition of lysosomal vacuolar proton pump down-regulates cellular acidification and enhances E. coli bactofection efficiency

Abstract

Keywords

Access to Document

Other files and links

Projects

Advancing E.coli as a Vector for Gene Delivery into Human Cells: Hybird with DNA /protein delivery reagents and intracellular strategies

Cite this