Inhibition of lysosomal vacuolar proton pump down-regulates cellular acidification and enhances E. coli bactofection efficiency

Rasaq Olajide Akinsola, Choon Weng Lee, Edmund Ui Hang Sim, Kumaran Narayanan

    Research output: Contribution to journalArticleOtherpeer-review

    Abstract

    Endosomal escape is considered a crucial barrier that needs to be overcome by integrin-mediated E. coli for gene delivery into mammalian cells. Bafilomycin, a potent inhibitor of the H+ proton pump commonly employed to lower endosomal pH, was evaluated as part of the E. coli protocol during delivery. We found an increase in green fluorescent protein expression up 6.9, 3.2, 5.0, 2.8, and 4.5 fold in HeLa, HEK-293, A549, HT1080, and MCF-7 respectively, compared to untreated cells. Our result showed for the first time that Inhibition of lysosomal V-ATPase enhances E. coli efficiency.

    Original languageEnglish
    Article number114088
    Number of pages3
    JournalAnalytical Biochemistry
    Volume616
    DOIs
    Publication statusPublished - 1 Mar 2021

    Keywords

    • Bacteria DNA delivery
    • Endocytosed
    • Endosome
    • Lysosome
    • Proton pump inhibitor
    • V-ATPase

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