TY - JOUR
T1 - Inhibition of interleukin 1β-converting enzyme-mediated apoptosis of mammalian cells by baculovirus IAP
AU - Hawkins, Christine J.
AU - Uren, Anthony G.
AU - Häcker, Georg
AU - Medcalf, Robert L.
AU - Vaux, David L.
PY - 1996/11/26
Y1 - 1996/11/26
N2 - Apoptosis can be a potent weapon against viral infection and consequently has selected for viruses carrying antiapoptosis genes. Two baculovirus proteins, IAP and p35, can prevent insect cells from dying in response to infection. p35, which interferes with members of the Ced-3 family of cysteine proteases, can also function in mammalian cells. We investigated the ability of IAP from Orgyia pseudotsugata nuclear polyhedrosis virus to prevent death of mammalian cells. IAP was transiently expressed in mammalian cells and its ability to block cell death caused by expression of interleukin-1β converting enzyme (ICE), FADD, or the ICE homologues ICH-I and ICE-Lap3, was investigated. IAP strongly inhibited ICE- and ICH-1-induced cell death but protected only partially against death by overexpression of FADD and not at all against death due to enforced ICE- Lap3 expression. These results demonstrate that a baculoviral IAP protein can functionally interact with conserved components of the apoptosis machinery in mammalian cells.
AB - Apoptosis can be a potent weapon against viral infection and consequently has selected for viruses carrying antiapoptosis genes. Two baculovirus proteins, IAP and p35, can prevent insect cells from dying in response to infection. p35, which interferes with members of the Ced-3 family of cysteine proteases, can also function in mammalian cells. We investigated the ability of IAP from Orgyia pseudotsugata nuclear polyhedrosis virus to prevent death of mammalian cells. IAP was transiently expressed in mammalian cells and its ability to block cell death caused by expression of interleukin-1β converting enzyme (ICE), FADD, or the ICE homologues ICH-I and ICE-Lap3, was investigated. IAP strongly inhibited ICE- and ICH-1-induced cell death but protected only partially against death by overexpression of FADD and not at all against death due to enforced ICE- Lap3 expression. These results demonstrate that a baculoviral IAP protein can functionally interact with conserved components of the apoptosis machinery in mammalian cells.
KW - FADD
KW - ICH-1
KW - interleukin 1β-converting enzyme
KW - interleukin 1β-converting enzyme- Lap3
UR - http://www.scopus.com/inward/record.url?scp=0030447149&partnerID=8YFLogxK
U2 - 10.1073/pnas.93.24.13786
DO - 10.1073/pnas.93.24.13786
M3 - Article
C2 - 8943013
AN - SCOPUS:0030447149
VL - 93
SP - 13786
EP - 13790
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
SN - 0027-8424
IS - 24
ER -