TY - JOUR
T1 - Inhibition of influenza A virus replication by short double-stranded oligodeoxynucleotides
AU - Kwok, Terry
AU - Helfer, Hanspeter
AU - Alam, M Intakhab
AU - Heinrich, Jochen
AU - Pavlovic, Jovan
AU - Moelling, Karin
PY - 2009
Y1 - 2009
N2 - Influenza A virus causes prevalent respiratory tract infections in humans. Small interfering RNA (siRNA) and antisense oligonucleotides (asODNs) have been used previously for silencing the RNA genome of influenza virus. Here, we explored the use of partially double-stranded oligodeoxynucleotides (dsODNs) to suppress the production of influenza A virus in cell cultures and animal models. We were able to inhibit influenza A virus replication in cultured human lung cells as well as in the lungs of infected C57BL/6 mice by treatment with dsODN 3-h post-infection. In about 20 of the cases (15/77) the titer was reduced by 10- to 100-fold and in 10 up to 1,000-fold. The antiviral effects of dsODNs were dose-dependent, sequence-dependent and comparable to those of its antisense and siRNA analogues. Thus, dsODNs may be developed as an additional class of nucleic acids for the inhibition of influenza virus replication.
AB - Influenza A virus causes prevalent respiratory tract infections in humans. Small interfering RNA (siRNA) and antisense oligonucleotides (asODNs) have been used previously for silencing the RNA genome of influenza virus. Here, we explored the use of partially double-stranded oligodeoxynucleotides (dsODNs) to suppress the production of influenza A virus in cell cultures and animal models. We were able to inhibit influenza A virus replication in cultured human lung cells as well as in the lungs of infected C57BL/6 mice by treatment with dsODN 3-h post-infection. In about 20 of the cases (15/77) the titer was reduced by 10- to 100-fold and in 10 up to 1,000-fold. The antiviral effects of dsODNs were dose-dependent, sequence-dependent and comparable to those of its antisense and siRNA analogues. Thus, dsODNs may be developed as an additional class of nucleic acids for the inhibition of influenza virus replication.
UR - http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=19034603
U2 - 10.1007/s00705-008-0262-z
DO - 10.1007/s00705-008-0262-z
M3 - Article
VL - 154
SP - 109
EP - 114
JO - Archives of Virology
JF - Archives of Virology
SN - 0304-8608
IS - 1
ER -