Inhibition of dynamin by dynole 34-2 induces cell death following cytokinesis failure in cancer cells

Megan Chircop, Swetha Perera, Anna Mariana, Hui Lau, Maggie P.C. Ma, Jayne Gilbert, Nigel C. Jones, Christopher P. Gordon, Kelly A. Young, Andrew Morokoff, Jennette Sakoff, Terence J. O'Brien, Adam McCluskey, Phillip J. Robinson

Research output: Contribution to journalArticleResearchpeer-review

33 Citations (Scopus)

Abstract

Inhibitors of mitotic proteins such as Aurora kinase and polo-like kinase have shown promise in preclinical or early clinical development for cancer treatment. We have reported that the MiTMAB class of dynamin small molecule inhibitors are new antimitotic agents with a novel mechanism of action, blocking cytokinesis. Here, we examined 5 of the most potent of a new series of dynamin GTPase inhibitors called dynoles. They all induced cytokinesis failure at the point of abscission, consistent with inhibition of dynamin while not affecting other cell cycle stages. All 5 dynoles inhibited cell proliferation (MTT and colony formation assays) in 11 cancer cell lines. The most potent GTPase inhibitor, dynole 34-2, also induced apoptosis, as revealed by cell blebbing, DNA fragmentation, and PARP cleavage. Cell death was induced specifically following cytokinesis failure, suggesting that dynole 34-2 selectively targets dividing cells. Dividing HeLa cells were more sensitive to the antiproliferative properties of all 5 dynoles compared with nondividing cells, and nontumorigenic fibroblasts were less sensitive to cell death induced by dynole 34-2. Thus, the dynoles are a second class of dynamin GTPase inhibitors, with dynole 34-2 as the lead compound, that are novel antimitotic compounds acting specifically at the abscission stage.

Original languageEnglish
Pages (from-to)1553-1562
Number of pages10
JournalMolecular Cancer Therapeutics
Volume10
Issue number9
DOIs
Publication statusPublished - Sep 2011
Externally publishedYes

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