Inhibition of Bak activation by VDAC2 is dependent on the Bak transmembrane anchor

Michael Lazarou, Diana Stojanovski, Ann E. Frazier, Aneta Kotevski, Grant Dewson, William J. Craigen, Ruth M. Kluck, David L. Vaux, Michael T. Ryan

Research output: Contribution to journalArticleResearchpeer-review

69 Citations (Scopus)

Abstract

Bax and Bak are pro-apoptotic factors that are required for cell death by the mitochondrial or intrinsic pathway. Bax is found in an inactive state in the cytosol and upon activation is targeted to the mitochondrial outer membrane where it releases cytochrome c and other factors that cause caspase activation. Although Bak functions in the same way as Bax, it is constitutively localized to the mitochondrial outer membrane. In the membrane, Bak activation is inhibited by the voltage-dependent anion channel isoform 2 (VDAC2) by an unknown mechanism. Using blue native gel electrophoresis, we show that in healthy cells endogenous inactive Bak exists in a 400-kDa complex that is dependent on the presence of VDAC2. Activation of Bak is concomitant with its release from the 400-kDa complex and the formation of lower molecular weight species. Furthermore, substitution of the Bak transmembrane anchor with that of the mitochondrial outer membrane tail-anchored protein hF is1 prevents association of Bak with the VDAC2 complex and increases the sensitivity of cells to an apoptotic stimulus. Our results suggest that VDAC2 interacts with the hydrophobic tail of Bak to sequester it in an inactive state in the mitochondrial outer membrane, thereby raising the stimulation threshold necessary for permeabilization of the mitochondrial outer membrane and cell death.

Original languageEnglish
Pages (from-to)36876-36883
Number of pages8
JournalJournal of Biological Chemistry
Volume285
Issue number47
DOIs
Publication statusPublished - 19 Nov 2010
Externally publishedYes

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