TY - JOUR
T1 - Infrared Based Saliva Screening Test for COVID-19
AU - Wood, Bayden R.
AU - Kochan, Kamila
AU - Bedolla, Diana E.
AU - Salazar-Quiroz, Natalia
AU - Grimley, Samantha L.
AU - Perez-Guaita, David
AU - Baker, Matthew J.
AU - Vongsvivut, Jitraporn
AU - Tobin, Mark J.
AU - Bambery, Keith R.
AU - Christensen, Dale
AU - Pasricha, Shivani
AU - Eden, Anthony K.
AU - Mclean, Aaron
AU - Roy, Supti
AU - Roberts, Jason A.
AU - Druce, Julian
AU - Williamson, Deborah A.
AU - McAuley, Julie
AU - Catton, Mike
AU - Purcell, Damian F.J.
AU - Godfrey, Dale I.
AU - Heraud, Philip
N1 - Funding Information:
Mr. Finlay Shanks for instrumental support. Dr Emanuele Pedersoli for his assistance in graphic scripting. Funding: Part of this research was undertaken on the IRM beamline at Australian Synchrotron (Victoria, Australia), part of the Australian Nuclear Science and Technology Organisation (ANSTO). We acknowledge the support of the beamtime (Proposal ID. 16460 and 16476) provided by ANSTO, funded by the Australian Government. This work was in‐part funded by Monash Green Chemical Futures Special Project fund. Part of this work was funded by the A2 milk foundation. Instruments were loaned and modified for transflection infrared slides by Dr Jerry Sellors and Dr Robert Packer courtesy of PerkinElmer®. N.S.Q, S.G. and J.M. were supported by funds from the Jack Ma Foundation through a grant to D.P. and D.I.G. D.P. and D.I.G. were supported by the National Health and Medical Research Council of Australia (NHMRC), including a Senior Principal Research Fellowship (1117766) and the Australian Research Council CE140100011 for D.I.G. D.P.‐G. acknowledges the financial support from the 2019 Ramón y Cajal (RYC) Contracts Aids (RYC2019‐026556‐I).
Funding Information:
Mr. Finlay Shanks for instrumental support. Dr Emanuele Pedersoli for his assistance in graphic scripting. Funding: Part of this research was undertaken on the IRM beamline at Australian Synchrotron (Victoria, Australia), part of the Australian Nuclear Science and Technology Organisation (ANSTO). We acknowledge the support of the beamtime (Proposal ID. 16460 and 16476) provided by ANSTO, funded by the Australian Government. This work was in-part funded by Monash Green Chemical Futures Special Project fund. Part of this work was funded by the A2 milk foundation. Instruments were loaned and modified for transflection infrared slides by Dr Jerry Sellors and Dr Robert Packer courtesy of PerkinElmer?. N.S.Q, S.G. and J.M. were supported by funds from the Jack Ma Foundation through a grant to D.P. and D.I.G. D.P. and D.I.G. were supported by the National Health and Medical Research Council of Australia (NHMRC), including a Senior Principal Research Fellowship (1117766) and the Australian Research Council CE140100011 for D.I.G. D.P.-G. acknowledges the financial support from the 2019 Ram?n y Cajal (RYC) Contracts Aids (RYC2019-026556-I).
Publisher Copyright:
© 2021 Wiley-VCH GmbH
PY - 2021/7/26
Y1 - 2021/7/26
N2 - Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has resulted in an unprecedented need for diagnostic testing that is critical in controlling the spread of COVID-19. We propose a portable infrared spectrometer with purpose-built transflection accessory for rapid point-of-care detection of COVID-19 markers in saliva. Initially, purified virion particles were characterized with Raman spectroscopy, synchrotron infrared (IR) and AFM-IR. A data set comprising 171 transflection infrared spectra from 29 subjects testing positive for SARS-CoV-2 by RT-qPCR and 28 testing negative, was modeled using Monte Carlo Double Cross Validation with 50 randomized test and model sets. The testing sensitivity was 93 % (27/29) with a specificity of 82 % (23/28) that included positive samples on the limit of detection for RT-qPCR. Herein, we demonstrate a proof-of-concept high throughput infrared COVID-19 test that is rapid, inexpensive, portable and utilizes sample self-collection thus minimizing the risk to healthcare workers and ideally suited to mass screening.
AB - Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has resulted in an unprecedented need for diagnostic testing that is critical in controlling the spread of COVID-19. We propose a portable infrared spectrometer with purpose-built transflection accessory for rapid point-of-care detection of COVID-19 markers in saliva. Initially, purified virion particles were characterized with Raman spectroscopy, synchrotron infrared (IR) and AFM-IR. A data set comprising 171 transflection infrared spectra from 29 subjects testing positive for SARS-CoV-2 by RT-qPCR and 28 testing negative, was modeled using Monte Carlo Double Cross Validation with 50 randomized test and model sets. The testing sensitivity was 93 % (27/29) with a specificity of 82 % (23/28) that included positive samples on the limit of detection for RT-qPCR. Herein, we demonstrate a proof-of-concept high throughput infrared COVID-19 test that is rapid, inexpensive, portable and utilizes sample self-collection thus minimizing the risk to healthcare workers and ideally suited to mass screening.
KW - COVID-19 diagnostic
KW - Fourier transform infrared (FTIR) spectroscopy
KW - Raman spectroscopy
KW - saliva
KW - SARS-CoV-2
UR - https://www.scopus.com/pages/publications/85108856948
U2 - 10.1002/anie.202104453
DO - 10.1002/anie.202104453
M3 - Article
C2 - 34043272
AN - SCOPUS:85108856948
SN - 1433-7851
VL - 60
SP - 17102
EP - 17107
JO - Angewandte Chemie - International Edition
JF - Angewandte Chemie - International Edition
IS - 31
ER -