Influenza A virus facilitates Streptococcus pneumoniae transmission and disease

Dimitri A Diavatopoulos, Kirsty R Short, John Timothy Price, Jonathan J Wilksch, Lorena E Brown, David E Briles, Richard A Strugnell, Odilia LC Wijburg

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Abstract

Streptococcus pneumoniae (the pneumococcus) kills approximately 1.6 million people annually. Pneumococcal infections predominantly manifest as pneumonia, sepsis, meningitis, and otitis media. S. pneumoniae is also a member of the normal nasopharyngeal flora, colonizing up to 80 of children. Infection with influenza A virus (IAV) has been associated with both pneumococcal disease and transmission. However, to date no animal model has been available to investigate the role of IAV in the spread of S. pneumoniae. Here we investigate pneumococcal-influenza synergism with a particular focus on the role of IAV on pneumococcal transmission. Infant mice were colonized with S. pneumoniae and subsequently infected with IAV 3 d later. Using this novel model we show increased pneumococcal colonization and disease in the presence of IAV. Notably, in vivo imaging showed that IAV was essential for the transmission of S. pneumoniae from colonized ( index ) mice to their naive cohoused littermates ( contacts ). Transmission occurred only when all mice were infected with IAV and was prevented when an IAV-neutralizing antibody was used to inhibit IAV replication in either index mice or contact mice. Together, these data provide novel insights into pneumococcal-influenza synergism and may indicate a previously unappreciated role of IAV in the spread of S. pneumoniae.
Original languageEnglish
Pages (from-to)1789 - 1798
Number of pages10
JournalFASEB Journal
Volume24
Issue number6
DOIs
Publication statusPublished - 2010

Cite this

Diavatopoulos, D. A., Short, K. R., Price, J. T., Wilksch, J. J., Brown, L. E., Briles, D. E., ... Wijburg, O. LC. (2010). Influenza A virus facilitates Streptococcus pneumoniae transmission and disease. FASEB Journal, 24(6), 1789 - 1798. https://doi.org/10.1096/fj.09-146779
Diavatopoulos, Dimitri A ; Short, Kirsty R ; Price, John Timothy ; Wilksch, Jonathan J ; Brown, Lorena E ; Briles, David E ; Strugnell, Richard A ; Wijburg, Odilia LC. / Influenza A virus facilitates Streptococcus pneumoniae transmission and disease. In: FASEB Journal. 2010 ; Vol. 24, No. 6. pp. 1789 - 1798.
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abstract = "Streptococcus pneumoniae (the pneumococcus) kills approximately 1.6 million people annually. Pneumococcal infections predominantly manifest as pneumonia, sepsis, meningitis, and otitis media. S. pneumoniae is also a member of the normal nasopharyngeal flora, colonizing up to 80 of children. Infection with influenza A virus (IAV) has been associated with both pneumococcal disease and transmission. However, to date no animal model has been available to investigate the role of IAV in the spread of S. pneumoniae. Here we investigate pneumococcal-influenza synergism with a particular focus on the role of IAV on pneumococcal transmission. Infant mice were colonized with S. pneumoniae and subsequently infected with IAV 3 d later. Using this novel model we show increased pneumococcal colonization and disease in the presence of IAV. Notably, in vivo imaging showed that IAV was essential for the transmission of S. pneumoniae from colonized ( index ) mice to their naive cohoused littermates ( contacts ). Transmission occurred only when all mice were infected with IAV and was prevented when an IAV-neutralizing antibody was used to inhibit IAV replication in either index mice or contact mice. Together, these data provide novel insights into pneumococcal-influenza synergism and may indicate a previously unappreciated role of IAV in the spread of S. pneumoniae.",
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Diavatopoulos, DA, Short, KR, Price, JT, Wilksch, JJ, Brown, LE, Briles, DE, Strugnell, RA & Wijburg, OLC 2010, 'Influenza A virus facilitates Streptococcus pneumoniae transmission and disease', FASEB Journal, vol. 24, no. 6, pp. 1789 - 1798. https://doi.org/10.1096/fj.09-146779

Influenza A virus facilitates Streptococcus pneumoniae transmission and disease. / Diavatopoulos, Dimitri A; Short, Kirsty R; Price, John Timothy; Wilksch, Jonathan J; Brown, Lorena E; Briles, David E; Strugnell, Richard A; Wijburg, Odilia LC.

In: FASEB Journal, Vol. 24, No. 6, 2010, p. 1789 - 1798.

Research output: Contribution to journalArticleResearchpeer-review

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AU - Diavatopoulos, Dimitri A

AU - Short, Kirsty R

AU - Price, John Timothy

AU - Wilksch, Jonathan J

AU - Brown, Lorena E

AU - Briles, David E

AU - Strugnell, Richard A

AU - Wijburg, Odilia LC

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N2 - Streptococcus pneumoniae (the pneumococcus) kills approximately 1.6 million people annually. Pneumococcal infections predominantly manifest as pneumonia, sepsis, meningitis, and otitis media. S. pneumoniae is also a member of the normal nasopharyngeal flora, colonizing up to 80 of children. Infection with influenza A virus (IAV) has been associated with both pneumococcal disease and transmission. However, to date no animal model has been available to investigate the role of IAV in the spread of S. pneumoniae. Here we investigate pneumococcal-influenza synergism with a particular focus on the role of IAV on pneumococcal transmission. Infant mice were colonized with S. pneumoniae and subsequently infected with IAV 3 d later. Using this novel model we show increased pneumococcal colonization and disease in the presence of IAV. Notably, in vivo imaging showed that IAV was essential for the transmission of S. pneumoniae from colonized ( index ) mice to their naive cohoused littermates ( contacts ). Transmission occurred only when all mice were infected with IAV and was prevented when an IAV-neutralizing antibody was used to inhibit IAV replication in either index mice or contact mice. Together, these data provide novel insights into pneumococcal-influenza synergism and may indicate a previously unappreciated role of IAV in the spread of S. pneumoniae.

AB - Streptococcus pneumoniae (the pneumococcus) kills approximately 1.6 million people annually. Pneumococcal infections predominantly manifest as pneumonia, sepsis, meningitis, and otitis media. S. pneumoniae is also a member of the normal nasopharyngeal flora, colonizing up to 80 of children. Infection with influenza A virus (IAV) has been associated with both pneumococcal disease and transmission. However, to date no animal model has been available to investigate the role of IAV in the spread of S. pneumoniae. Here we investigate pneumococcal-influenza synergism with a particular focus on the role of IAV on pneumococcal transmission. Infant mice were colonized with S. pneumoniae and subsequently infected with IAV 3 d later. Using this novel model we show increased pneumococcal colonization and disease in the presence of IAV. Notably, in vivo imaging showed that IAV was essential for the transmission of S. pneumoniae from colonized ( index ) mice to their naive cohoused littermates ( contacts ). Transmission occurred only when all mice were infected with IAV and was prevented when an IAV-neutralizing antibody was used to inhibit IAV replication in either index mice or contact mice. Together, these data provide novel insights into pneumococcal-influenza synergism and may indicate a previously unappreciated role of IAV in the spread of S. pneumoniae.

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EP - 1798

JO - FASEB Journal

JF - FASEB Journal

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Diavatopoulos DA, Short KR, Price JT, Wilksch JJ, Brown LE, Briles DE et al. Influenza A virus facilitates Streptococcus pneumoniae transmission and disease. FASEB Journal. 2010;24(6):1789 - 1798. https://doi.org/10.1096/fj.09-146779