Influence of codon usage on the immunogenicity of a DNA vaccine against tetanus

Richard Stratford, Gillian Douce, Li Zhang-Barber, Neil Fairweather, Juhani Eskola, Gordon Dougan

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50 Citations (Scopus)

Abstract

Two related DNA vaccine vector plasmids, harbouring either wild-type (pcDNA3/ntetC) or synthetic codon optimised (pcDNA3/stetC) DNA encoding fragment C (TetC) of tetanus toxin were constructed. COS-7 cells transformed with pcDNA3/stetC reproducibly expressed higher levels of TetC than similar cells transformed with pcDNA3/ntetC. BALB/c mice immunised intramuscularly with pcDNA3/stetC produced significantly higher levels of anti-TetC antibodies in their serum in the weeks following vaccination compared to mice immunised with pcDNA3/ntetC, even when differences in the CpG content between the two sequences were controlled for using non-expressing DNA. Copyright (C) 2000 Elsevier Science Ltd.

Original languageEnglish
Pages (from-to)810-815
Number of pages6
JournalVaccine
Volume19
Issue number7-8
DOIs
Publication statusPublished - 22 Nov 2000
Externally publishedYes

Keywords

  • Codon usage
  • DNA vaccines
  • Murine model
  • Tetanus

Cite this

Stratford, R., Douce, G., Zhang-Barber, L., Fairweather, N., Eskola, J., & Dougan, G. (2000). Influence of codon usage on the immunogenicity of a DNA vaccine against tetanus. Vaccine, 19(7-8), 810-815. https://doi.org/10.1016/S0264-410X(00)00246-2