Inflight Polymerase Chain Reaction of samples with drones: inflight PCR with drones

Hassan Ali Abid; Tristan Minifie; Jian Wern Ong; Eric Shen Lin; Zhixiong Song; Oi Wah Liew; Tuck Wah Ng

doi:10.1016/j.ab.2020.114098

Inflight Polymerase Chain Reaction of samples with drones: inflight PCR with drones

Hassan Ali Abid, Tristan Minifie, Jian Wern Ong, Eric Shen Lin, Zhixiong Song, Oi Wah Liew, Tuck Wah Ng

Mechanical & Aerospace Engineering

Research output: Contribution to journal › Article › Research › peer-review

2 Citations (Scopus)

Abstract

A system devised to conduct Polymerase Chain Reaction (PCR) in-flight on drones that uses the spatial displacement of capillary tubes on thermal blocks kept at 94 °C, 58 °C and 72 °C corresponding to cycling temperatures for denaturation, annealing and extension is demonstrated here. The use of acetal as the thermal block material reduced heat loss and the input power (within 18.5 W) needed to maintain the required temperatures. Tests showed that concentrations of samples down to 1.16 × 10⁶ DNA copies/μL could be significantly and consistently detected above the background emission of the fluorescence signal intensity.

Original language	English
Article number	114098
Number of pages	3
Journal	Analytical Biochemistry
Volume	616
DOIs	https://doi.org/10.1016/j.ab.2020.114098
Publication status	Published - 1 Mar 2021

Keywords

Drone
PCR
Quadcopter
Transport
UAV

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10.1016/j.ab.2020.114098

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title = "Inflight Polymerase Chain Reaction of samples with drones: inflight PCR with drones",

abstract = "A system devised to conduct Polymerase Chain Reaction (PCR) in-flight on drones that uses the spatial displacement of capillary tubes on thermal blocks kept at 94 °C, 58 °C and 72 °C corresponding to cycling temperatures for denaturation, annealing and extension is demonstrated here. The use of acetal as the thermal block material reduced heat loss and the input power (within 18.5 W) needed to maintain the required temperatures. Tests showed that concentrations of samples down to 1.16 × 106 DNA copies/μL could be significantly and consistently detected above the background emission of the fluorescence signal intensity.",

keywords = "Drone, PCR, Quadcopter, Transport, UAV",

author = "Abid, {Hassan Ali} and Tristan Minifie and Ong, {Jian Wern} and Lin, {Eric Shen} and Zhixiong Song and Liew, {Oi Wah} and Ng, {Tuck Wah}",

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AU - Abid, Hassan Ali

AU - Minifie, Tristan

AU - Ong, Jian Wern

AU - Lin, Eric Shen

AU - Song, Zhixiong

AU - Liew, Oi Wah

AU - Ng, Tuck Wah

PY - 2021/3/1

Y1 - 2021/3/1

N2 - A system devised to conduct Polymerase Chain Reaction (PCR) in-flight on drones that uses the spatial displacement of capillary tubes on thermal blocks kept at 94 °C, 58 °C and 72 °C corresponding to cycling temperatures for denaturation, annealing and extension is demonstrated here. The use of acetal as the thermal block material reduced heat loss and the input power (within 18.5 W) needed to maintain the required temperatures. Tests showed that concentrations of samples down to 1.16 × 106 DNA copies/μL could be significantly and consistently detected above the background emission of the fluorescence signal intensity.

AB - A system devised to conduct Polymerase Chain Reaction (PCR) in-flight on drones that uses the spatial displacement of capillary tubes on thermal blocks kept at 94 °C, 58 °C and 72 °C corresponding to cycling temperatures for denaturation, annealing and extension is demonstrated here. The use of acetal as the thermal block material reduced heat loss and the input power (within 18.5 W) needed to maintain the required temperatures. Tests showed that concentrations of samples down to 1.16 × 106 DNA copies/μL could be significantly and consistently detected above the background emission of the fluorescence signal intensity.

KW - Drone

KW - PCR

KW - Quadcopter

KW - Transport

KW - UAV

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VL - 616

JO - Analytical Biochemistry

JF - Analytical Biochemistry

M1 - 114098

ER -

Inflight Polymerase Chain Reaction of samples with drones: inflight PCR with drones

Abstract

Keywords

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