Projects per year
Abstract
Identification of biomarkers in clinical applications for diagnostics at the point-of-care (POC) setting requires the development of industry viable biosensing platform. Herein, we report such development of biosensor architecture for the detection of pharmacogenetic biomarker HLA-B∗15:02 gene. The biosensor architecture comprises of an oligonucleotide stem-loop probe modified with a methylene blue redox (MB) reporter, immobilized via a rapid "printing"method on the commercially available disposable screen-printed electrodes (SPE). The square wave voltammetric measurements on the DNA sensor showed a clear peak difference of ∼80 nA with a significant difference in peak height values of the faradaic current generated for the MB redox moiety between the positive control (biotin-modified 19 based oligonucleotides with the sequence mimicking the specific region of the HLA-B∗15:02 allele and complementary to the probe sequence) and negative control samples (biotin-modified 19 based oligonucleotides with the sequence unrelated to the probe sequence and the HLA-B∗15:02 allele). These initial proof of concept results provide support for the possibility of using this signal-off biosensor architecture in the intended pharmacogenetic biomarker testing.
Original language | English |
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Article number | 017508 |
Number of pages | 6 |
Journal | Journal of the Electrochemical Society |
Volume | 169 |
Issue number | 1 |
DOIs | |
Publication status | Published - Jan 2022 |
Projects
- 1 Finished
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Precision medicine for epilepsy and beyond: from discovery to implementation and evaluation
1/01/18 → 31/12/22
Project: Research