Induction of peptide‐specific primary cytotoxic T lymphocyte responses from human peripheral blood

Magdalena Plebanski, Catherine E M Allsopp, Michael Aidoo, Hugh Reyburn, Adrian V S Hill

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52 Citations (Scopus)

Abstract

Various protocols were developed and compared for eliciting specific cytotoxic T lymphocyte (CTL) cell lines from the unselected human peripheral blood mononuclear cells of naive donors. Interleukin‐7 and CD4+ T cells primed in vitro by keyhole limpet hemocyanin were shown to act together in the generation of these responses. Primary responses were consistently induced with a variety of different HLA class I‐binding malarial peptides. Primary CTL responses could be induced from unselected CD8+ and from CD45RA+CD8+ T cells. The CTL lines derived from these naive donors were CD8+ and demonstrated a high level of HLA class I‐restricted killing for > 3 months after priming in vitro. They were also able to recognize and kill targets infected with a recombinant vaccinia virus containing the full‐length antigen. In addition, this same protocol enhanced up to fourfold the levels of secondary CTL responses induced. The optimal method presented for naive cytotoxic T cell stimulation is simple, rapid and generally applicable and should provide a useful tool for both basic research and human therapy.

Original languageEnglish
Pages (from-to)1783-1787
Number of pages5
JournalEuropean Journal of Immunology
Volume25
Issue number6
DOIs
Publication statusPublished - 1995
Externally publishedYes

Keywords

  • CD45RA
  • Cytotoxic T lymphocyte
  • Malaria
  • Peptide
  • Primary immune response

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