Induction of memory cytotoxic T cells to influenza A virus and subsequent viral clearance is not modulated by PB1-F2-dependent inflammasome activation

Patricia Y. Lee, Nicola L Bird, Charley MacKenzie-Kludas, Ashley Mansell, Katherine Kedzierska, Lorena Brown, Julie McAuley

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Expression of the viral virulence protein PB1-F2 during infection has been linked to NLRP3 inflammasome complex activation in macrophages and induction of early inflammatory events enhancing immunopathology during influenza disease. We sought to determine whether PB1-F2-specific NLRP3 inflammasome activation influenced the magnitude and/or robustness of the CD8+ T-cell responses specific for conserved viral antigens and subsequent virus elimination. Using murine heterosubtypic viral infection models, we showed that mice infected with virus unable to produce PB1-F2 protein showed no deficit in the overall magnitude and functional memory responses of CD8+ T cells established during the effector phase compared with those infected with wild-type PB1-F2-expressing virus and were equally capable of mounting robust recall responses. These data indicate that while expression of PB1-F2 protein can induce inflammatory events, the capacity to generate memory CD8+ T cells specific for immunodominant viral epitopes remains uncompromised.

Original languageEnglish
Pages (from-to)439-446
Number of pages8
JournalImmunology and Cell Biology
Volume94
Issue number5
DOIs
Publication statusPublished - 1 May 2016
Externally publishedYes

Cite this

Lee, Patricia Y. ; Bird, Nicola L ; MacKenzie-Kludas, Charley ; Mansell, Ashley ; Kedzierska, Katherine ; Brown, Lorena ; McAuley, Julie. / Induction of memory cytotoxic T cells to influenza A virus and subsequent viral clearance is not modulated by PB1-F2-dependent inflammasome activation. In: Immunology and Cell Biology. 2016 ; Vol. 94, No. 5. pp. 439-446.
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abstract = "Expression of the viral virulence protein PB1-F2 during infection has been linked to NLRP3 inflammasome complex activation in macrophages and induction of early inflammatory events enhancing immunopathology during influenza disease. We sought to determine whether PB1-F2-specific NLRP3 inflammasome activation influenced the magnitude and/or robustness of the CD8+ T-cell responses specific for conserved viral antigens and subsequent virus elimination. Using murine heterosubtypic viral infection models, we showed that mice infected with virus unable to produce PB1-F2 protein showed no deficit in the overall magnitude and functional memory responses of CD8+ T cells established during the effector phase compared with those infected with wild-type PB1-F2-expressing virus and were equally capable of mounting robust recall responses. These data indicate that while expression of PB1-F2 protein can induce inflammatory events, the capacity to generate memory CD8+ T cells specific for immunodominant viral epitopes remains uncompromised.",
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Induction of memory cytotoxic T cells to influenza A virus and subsequent viral clearance is not modulated by PB1-F2-dependent inflammasome activation. / Lee, Patricia Y.; Bird, Nicola L; MacKenzie-Kludas, Charley; Mansell, Ashley; Kedzierska, Katherine; Brown, Lorena; McAuley, Julie.

In: Immunology and Cell Biology, Vol. 94, No. 5, 01.05.2016, p. 439-446.

Research output: Contribution to journalArticleResearchpeer-review

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