Induction of angiogenesis by airway smooth muscle from patients with asthma

D E Simcock, V Kanabar, Graham W Clarke, K Mahn, C Karner, B J O'Connor, Tak H Lee, Stuart John Hirst

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Abstract

RATIONALE: Airway remodeling in asthma involves accumulation of airway smooth muscle (ASM) and increased vascularity due to angiogenesis. Bronchial blood vessels and ASM are found in close proximity, and ASM releases multiple proinflammatory mediators including vascular endothelial growth factor (VEGF). OBJECTIVE: We examined if release of proangiogenic mediators is increased in ASM from subjects with asthma and if this is translated to induction of angiogenesis. METHODS: Biopsy-derived ASM cells were cultured from 12 subjects with mild asthma, 8 with moderate asthma, and 9 healthy controls. Angiogenesis induced by cell-conditioned medium (CM) from ASM was evaluated in a tubule formation assay. Anti-CD31 labeled tubules were quantified by image analysis. Angiogenic factors in CM were quantified by antibody arrays and by ELISA. MEASUREMENTS AND MAIN RESULTS: Induction of angiogenesis by CM from unstimulated ASM was increased in subjects with mild asthma (2-fold) and moderate asthma (3-fold), compared with healthy CM (p
Original languageEnglish
Pages (from-to)461 - 468
Number of pages8
JournalAmerican Journal of Respiratory and Critical Care Medicine
Volume178
Issue number5
Publication statusPublished - 2008

Cite this

Simcock, D. E., Kanabar, V., Clarke, G. W., Mahn, K., Karner, C., O'Connor, B. J., Lee, T. H., & Hirst, S. J. (2008). Induction of angiogenesis by airway smooth muscle from patients with asthma. American Journal of Respiratory and Critical Care Medicine, 178(5), 461 - 468.