Inducing pluripotency in cattle

Luis Fernando Malaver Ortega, Amir Taheri-Ghahfarokhi, Huseyin Sumer

Research output: Chapter in Book/Report/Conference proceedingChapter (Book)Otherpeer-review

Abstract

Nuclear reprogramming technologies in general and induced pluripotent stem cells (iPSCs) in particular have opened the door to a vast number of practical applications in regenerative medicine and biotechnology. It also represents a possible alternative to the still evasive achievement of embryonic stem cells (ESCs) isolation from refractory species such as Bos. taurus. Herein, we described a protocol for bovine iPSCs (biPSCs) generation and characterization. The protocol is based on the overexpression of the exogenous transcription factors NANOG, OCT4, SOX2, KLF4 and c-MYC, using a pantropic retroviral system.
Original languageEnglish
Title of host publicationCell Reprogramming
Subtitle of host publicationMethods and Protocols
EditorsPaul J Verma, Huseyin Sumer
Place of PublicationNew York NY USA
PublisherHumana Press
Pages57-68
Number of pages12
Edition1st
ISBN (Print)9781493928477
DOIs
Publication statusPublished - 2015

Publication series

NameMethods in Molecular Biology: Springer Protocols
PublisherSpringer
Number1330
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Cite this

Malaver Ortega, L. F., Taheri-Ghahfarokhi, A., & Sumer, H. (2015). Inducing pluripotency in cattle. In P. J. Verma, & H. Sumer (Eds.), Cell Reprogramming: Methods and Protocols (1st ed., pp. 57-68). (Methods in Molecular Biology: Springer Protocols; No. 1330). New York NY USA: Humana Press. https://doi.org/10.1007/978-1-4030-2848-4_6
Malaver Ortega, Luis Fernando ; Taheri-Ghahfarokhi, Amir ; Sumer, Huseyin. / Inducing pluripotency in cattle. Cell Reprogramming: Methods and Protocols. editor / Paul J Verma ; Huseyin Sumer. 1st. ed. New York NY USA : Humana Press, 2015. pp. 57-68 (Methods in Molecular Biology: Springer Protocols; 1330).
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Malaver Ortega, LF, Taheri-Ghahfarokhi, A & Sumer, H 2015, Inducing pluripotency in cattle. in PJ Verma & H Sumer (eds), Cell Reprogramming: Methods and Protocols. 1st edn, Methods in Molecular Biology: Springer Protocols, no. 1330, Humana Press, New York NY USA, pp. 57-68. https://doi.org/10.1007/978-1-4030-2848-4_6

Inducing pluripotency in cattle. / Malaver Ortega, Luis Fernando; Taheri-Ghahfarokhi, Amir; Sumer, Huseyin.

Cell Reprogramming: Methods and Protocols. ed. / Paul J Verma; Huseyin Sumer. 1st. ed. New York NY USA : Humana Press, 2015. p. 57-68 (Methods in Molecular Biology: Springer Protocols; No. 1330).

Research output: Chapter in Book/Report/Conference proceedingChapter (Book)Otherpeer-review

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AU - Sumer, Huseyin

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N2 - Nuclear reprogramming technologies in general and induced pluripotent stem cells (iPSCs) in particular have opened the door to a vast number of practical applications in regenerative medicine and biotechnology. It also represents a possible alternative to the still evasive achievement of embryonic stem cells (ESCs) isolation from refractory species such as Bos. taurus. Herein, we described a protocol for bovine iPSCs (biPSCs) generation and characterization. The protocol is based on the overexpression of the exogenous transcription factors NANOG, OCT4, SOX2, KLF4 and c-MYC, using a pantropic retroviral system.

AB - Nuclear reprogramming technologies in general and induced pluripotent stem cells (iPSCs) in particular have opened the door to a vast number of practical applications in regenerative medicine and biotechnology. It also represents a possible alternative to the still evasive achievement of embryonic stem cells (ESCs) isolation from refractory species such as Bos. taurus. Herein, we described a protocol for bovine iPSCs (biPSCs) generation and characterization. The protocol is based on the overexpression of the exogenous transcription factors NANOG, OCT4, SOX2, KLF4 and c-MYC, using a pantropic retroviral system.

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Malaver Ortega LF, Taheri-Ghahfarokhi A, Sumer H. Inducing pluripotency in cattle. In Verma PJ, Sumer H, editors, Cell Reprogramming: Methods and Protocols. 1st ed. New York NY USA: Humana Press. 2015. p. 57-68. (Methods in Molecular Biology: Springer Protocols; 1330). https://doi.org/10.1007/978-1-4030-2848-4_6