Inducing pluripotency in cattle

Luis Fernando Malaver Ortega, Amir Taheri-Ghahfarokhi, Huseyin Sumer

Research output: Chapter in Book/Report/Conference proceedingChapter (Book)Otherpeer-review


Nuclear reprogramming technologies in general and induced pluripotent stem cells (iPSCs) in particular have opened the door to a vast number of practical applications in regenerative medicine and biotechnology. It also represents a possible alternative to the still evasive achievement of embryonic stem cells (ESCs) isolation from refractory species such as Bos. taurus. Herein, we described a protocol for bovine iPSCs (biPSCs) generation and characterization. The protocol is based on the overexpression of the exogenous transcription factors NANOG, OCT4, SOX2, KLF4 and c-MYC, using a pantropic retroviral system.
Original languageEnglish
Title of host publicationCell Reprogramming
Subtitle of host publicationMethods and Protocols
EditorsPaul J Verma, Huseyin Sumer
Place of PublicationNew York NY USA
PublisherHumana Press
Number of pages12
ISBN (Print)9781493928477
Publication statusPublished - 2015

Publication series

NameMethods in Molecular Biology: Springer Protocols
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

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