Inducing pluripotency in cattle

Luis Fernando Malaver Ortega; Amir Taheri-Ghahfarokhi; Huseyin Sumer

doi:10.1007/978-1-4030-2848-4_6

Inducing pluripotency in cattle

Luis Fernando Malaver Ortega, Amir Taheri-Ghahfarokhi, Huseyin Sumer

Research output: Chapter in Book/Report/Conference proceeding › Chapter (Book) › Other › peer-review

Abstract

Nuclear reprogramming technologies in general and induced pluripotent stem cells (iPSCs) in particular have opened the door to a vast number of practical applications in regenerative medicine and biotechnology. It also represents a possible alternative to the still evasive achievement of embryonic stem cells (ESCs) isolation from refractory species such as Bos. taurus. Herein, we described a protocol for bovine iPSCs (biPSCs) generation and characterization. The protocol is based on the overexpression of the exogenous transcription factors NANOG, OCT4, SOX2, KLF4 and c-MYC, using a pantropic retroviral system.

Original language	English
Title of host publication	Cell Reprogramming
Subtitle of host publication	Methods and Protocols
Editors	Paul J Verma, Huseyin Sumer
Place of Publication	New York NY USA
Publisher	Humana Press
Pages	57-68
Number of pages	12
Edition	1st
ISBN (Print)	9781493928477
DOIs	https://doi.org/10.1007/978-1-4030-2848-4_6
Publication status	Published - 2015

Publication series

Name	Methods in Molecular Biology: Springer Protocols
Publisher	Springer
Number	1330
ISSN (Print)	1064-3745
ISSN (Electronic)	1940-6029

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10.1007/978-1-4030-2848-4_6

Cite this

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abstract = "Nuclear reprogramming technologies in general and induced pluripotent stem cells (iPSCs) in particular have opened the door to a vast number of practical applications in regenerative medicine and biotechnology. It also represents a possible alternative to the still evasive achievement of embryonic stem cells (ESCs) isolation from refractory species such as Bos. taurus. Herein, we described a protocol for bovine iPSCs (biPSCs) generation and characterization. The protocol is based on the overexpression of the exogenous transcription factors NANOG, OCT4, SOX2, KLF4 and c-MYC, using a pantropic retroviral system.",

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Inducing pluripotency in cattle. / Malaver Ortega, Luis Fernando; Taheri-Ghahfarokhi, Amir; Sumer, Huseyin.
Cell Reprogramming: Methods and Protocols. ed. / Paul J Verma; Huseyin Sumer. 1st. ed. New York NY USA: Humana Press, 2015. p. 57-68 (Methods in Molecular Biology: Springer Protocols; No. 1330).

Research output: Chapter in Book/Report/Conference proceeding › Chapter (Book) › Other › peer-review

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AU - Malaver Ortega, Luis Fernando

AU - Taheri-Ghahfarokhi, Amir

AU - Sumer, Huseyin

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N2 - Nuclear reprogramming technologies in general and induced pluripotent stem cells (iPSCs) in particular have opened the door to a vast number of practical applications in regenerative medicine and biotechnology. It also represents a possible alternative to the still evasive achievement of embryonic stem cells (ESCs) isolation from refractory species such as Bos. taurus. Herein, we described a protocol for bovine iPSCs (biPSCs) generation and characterization. The protocol is based on the overexpression of the exogenous transcription factors NANOG, OCT4, SOX2, KLF4 and c-MYC, using a pantropic retroviral system.

AB - Nuclear reprogramming technologies in general and induced pluripotent stem cells (iPSCs) in particular have opened the door to a vast number of practical applications in regenerative medicine and biotechnology. It also represents a possible alternative to the still evasive achievement of embryonic stem cells (ESCs) isolation from refractory species such as Bos. taurus. Herein, we described a protocol for bovine iPSCs (biPSCs) generation and characterization. The protocol is based on the overexpression of the exogenous transcription factors NANOG, OCT4, SOX2, KLF4 and c-MYC, using a pantropic retroviral system.

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A2 - Sumer, Huseyin

PB - Humana Press

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ER -

Inducing pluripotency in cattle

Abstract

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