Inducing and Inhibiting Autophagy to Investigate Its Interactions with MIF

Nadia S. Deen, Jacinta P. Lee, James Harris

Research output: Chapter in Book/Report/Conference proceedingChapter (Book)Otherpeer-review

Abstract

MIF has been described to be associated with autophagy in a number of studies, but the full nature of their association is not yet clear. However, the unprecedented interest in autophagy in recent times has generated a number of tools and techniques for its study. Here, we present protocols for studying the interactions between MIF and autophagy, including for the induction and inhibition of autophagy and measuring autophagosome biogenesis and maturation.

Original languageEnglish
Title of host publicationMacrophage Migration Inhibitory Factor
Subtitle of host publicationMethods and Protocols
EditorsJames Harris, Eric Morand
Place of PublicationNew York, NY
PublisherHumana Press
Chapter13
Pages147-158
Number of pages12
ISBN (Electronic)9781493999361
ISBN (Print)9781493999354
DOIs
Publication statusPublished - 1 Jan 2020

Publication series

NameMethods in Molecular Biology: Springer Protocols
PublisherSpringer
Volume2080
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • Autophagosome
  • LC3
  • Macroautophagy
  • Rapamycin
  • siRNA
  • SQSTM1/p62

Cite this

Deen, N. S., Lee, J. P., & Harris, J. (2020). Inducing and Inhibiting Autophagy to Investigate Its Interactions with MIF. In J. Harris, & E. Morand (Eds.), Macrophage Migration Inhibitory Factor: Methods and Protocols (pp. 147-158). (Methods in Molecular Biology: Springer Protocols; Vol. 2080). New York, NY: Humana Press. https://doi.org/10.1007/978-1-4939-9936-1_13