Inducing and Inhibiting Autophagy to Investigate Its Interactions with MIF

Nadia S. Deen; Jacinta P. Lee; James Harris

doi:10.1007/978-1-4939-9936-1_13

Inducing and Inhibiting Autophagy to Investigate Its Interactions with MIF

Nadia S. Deen, Jacinta P. Lee, James Harris

Centre for Inflammatory Disease Monash Health

Research output: Chapter in Book/Report/Conference proceeding › Chapter (Book) › Other › peer-review

Abstract

MIF has been described to be associated with autophagy in a number of studies, but the full nature of their association is not yet clear. However, the unprecedented interest in autophagy in recent times has generated a number of tools and techniques for its study. Here, we present protocols for studying the interactions between MIF and autophagy, including for the induction and inhibition of autophagy and measuring autophagosome biogenesis and maturation.

Original language	English
Title of host publication	Macrophage Migration Inhibitory Factor
Subtitle of host publication	Methods and Protocols
Editors	James Harris, Eric Morand
Place of Publication	New York, NY
Publisher	Humana Press
Chapter	13
Pages	147-158
Number of pages	12
ISBN (Electronic)	9781493999361
ISBN (Print)	9781493999354
DOIs	https://doi.org/10.1007/978-1-4939-9936-1_13
Publication status	Published - 1 Jan 2020

Publication series

Name	Methods in Molecular Biology: Springer Protocols
Publisher	Springer
Volume	2080
ISSN (Print)	1064-3745
ISSN (Electronic)	1940-6029

Keywords

Autophagosome
LC3
Macroautophagy
Rapamycin
siRNA
SQSTM1/p62

Cite this

Deen, N. S., Lee, J. P., & Harris, J. (2020). Inducing and Inhibiting Autophagy to Investigate Its Interactions with MIF. In J. Harris, & E. Morand (Eds.), Macrophage Migration Inhibitory Factor: Methods and Protocols (pp. 147-158). (Methods in Molecular Biology: Springer Protocols; Vol. 2080). New York, NY: Humana Press. https://doi.org/10.1007/978-1-4939-9936-1_13

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Inducing and Inhibiting Autophagy to Investigate Its Interactions with MIF. / Deen, Nadia S.; Lee, Jacinta P.; Harris, James.

Macrophage Migration Inhibitory Factor: Methods and Protocols. ed. / James Harris; Eric Morand. New York, NY : Humana Press, 2020. p. 147-158 (Methods in Molecular Biology: Springer Protocols; Vol. 2080).

Research output: Chapter in Book/Report/Conference proceeding › Chapter (Book) › Other › peer-review

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Access to Document

10.1007/978-1-4939-9936-1_13

Link to publication in Scopus