Independent regulation of vertebral number and vertebral identity by microRNA-196 paralogs

S F Lisa Wong, Vikram Agarwal, Jennifer H Mansfield, Nicolas Denans, Matthew G Schwartz, Haydn M Prosser, Olivier Pourquie, David P Bartel, Clifford J Tabin, Edwina McGlinn

Research output: Contribution to journalArticleResearchpeer-review

Abstract

The Hox genes play a central role in patterning the embryonic anterior-to-posterior axis. An important function of Hox activity in vertebrates is the specification of different vertebral morphologies, with an additional role in axis elongation emerging. The miR-196 family of microRNAs (miRNAs) are predicted to extensively target Hox 3 UTRs, although the full extent to which miR-196 regulates Hox expression dynamics and influences mammalian development remains to be elucidated. Here we used an extensive allelic series of mouse knockouts to show that the miR-196 family of miRNAs is essential both for properly patterning vertebral identity at different axial levels and for modulating the total number of vertebrae. All three miR-196 paralogs, 196a1, 196a2, and 196b, act redundantly to pattern the midthoracic region, whereas 196a2 and 196b have an additive role in controlling the number of rib-bearing vertebra and positioning of the sacrum. Independent of this, 196a1, 196a2, and 196b act redundantly to constrain total vertebral number. Loss of miR-196 leads to a collective up-regulation of numerous trunk Hox target genes with a concomitant delay in activation of caudal Hox genes, which are proposed to signal the end of axis extension. Additionally, we identified altered molecular signatures associated with the Wnt, Fgf, and Notch/segmentation pathways and demonstrate that miR-196 has the potential to regulate Wnt activity by multiple mechanisms. By feeding into, and thereby integrating, multiple genetic networks controlling vertebral number and identity, miR-196 is a critical player defining axial formulae.
Original languageEnglish
Pages (from-to)4884 - 4893
Number of pages10
JournalProceedings of the National Academy of Sciences
Volume112
Issue number35
DOIs
Publication statusPublished - 2015

Cite this

Wong, S F Lisa ; Agarwal, Vikram ; Mansfield, Jennifer H ; Denans, Nicolas ; Schwartz, Matthew G ; Prosser, Haydn M ; Pourquie, Olivier ; Bartel, David P ; Tabin, Clifford J ; McGlinn, Edwina. / Independent regulation of vertebral number and vertebral identity by microRNA-196 paralogs. In: Proceedings of the National Academy of Sciences. 2015 ; Vol. 112, No. 35. pp. 4884 - 4893.
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abstract = "The Hox genes play a central role in patterning the embryonic anterior-to-posterior axis. An important function of Hox activity in vertebrates is the specification of different vertebral morphologies, with an additional role in axis elongation emerging. The miR-196 family of microRNAs (miRNAs) are predicted to extensively target Hox 3 UTRs, although the full extent to which miR-196 regulates Hox expression dynamics and influences mammalian development remains to be elucidated. Here we used an extensive allelic series of mouse knockouts to show that the miR-196 family of miRNAs is essential both for properly patterning vertebral identity at different axial levels and for modulating the total number of vertebrae. All three miR-196 paralogs, 196a1, 196a2, and 196b, act redundantly to pattern the midthoracic region, whereas 196a2 and 196b have an additive role in controlling the number of rib-bearing vertebra and positioning of the sacrum. Independent of this, 196a1, 196a2, and 196b act redundantly to constrain total vertebral number. Loss of miR-196 leads to a collective up-regulation of numerous trunk Hox target genes with a concomitant delay in activation of caudal Hox genes, which are proposed to signal the end of axis extension. Additionally, we identified altered molecular signatures associated with the Wnt, Fgf, and Notch/segmentation pathways and demonstrate that miR-196 has the potential to regulate Wnt activity by multiple mechanisms. By feeding into, and thereby integrating, multiple genetic networks controlling vertebral number and identity, miR-196 is a critical player defining axial formulae.",
author = "Wong, {S F Lisa} and Vikram Agarwal and Mansfield, {Jennifer H} and Nicolas Denans and Schwartz, {Matthew G} and Prosser, {Haydn M} and Olivier Pourquie and Bartel, {David P} and Tabin, {Clifford J} and Edwina McGlinn",
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language = "English",
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Wong, SFL, Agarwal, V, Mansfield, JH, Denans, N, Schwartz, MG, Prosser, HM, Pourquie, O, Bartel, DP, Tabin, CJ & McGlinn, E 2015, 'Independent regulation of vertebral number and vertebral identity by microRNA-196 paralogs' Proceedings of the National Academy of Sciences, vol. 112, no. 35, pp. 4884 - 4893. https://doi.org/10.1073/pnas.1512655112

Independent regulation of vertebral number and vertebral identity by microRNA-196 paralogs. / Wong, S F Lisa; Agarwal, Vikram; Mansfield, Jennifer H; Denans, Nicolas; Schwartz, Matthew G; Prosser, Haydn M; Pourquie, Olivier; Bartel, David P; Tabin, Clifford J; McGlinn, Edwina.

In: Proceedings of the National Academy of Sciences, Vol. 112, No. 35, 2015, p. 4884 - 4893.

Research output: Contribution to journalArticleResearchpeer-review

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T1 - Independent regulation of vertebral number and vertebral identity by microRNA-196 paralogs

AU - Wong, S F Lisa

AU - Agarwal, Vikram

AU - Mansfield, Jennifer H

AU - Denans, Nicolas

AU - Schwartz, Matthew G

AU - Prosser, Haydn M

AU - Pourquie, Olivier

AU - Bartel, David P

AU - Tabin, Clifford J

AU - McGlinn, Edwina

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N2 - The Hox genes play a central role in patterning the embryonic anterior-to-posterior axis. An important function of Hox activity in vertebrates is the specification of different vertebral morphologies, with an additional role in axis elongation emerging. The miR-196 family of microRNAs (miRNAs) are predicted to extensively target Hox 3 UTRs, although the full extent to which miR-196 regulates Hox expression dynamics and influences mammalian development remains to be elucidated. Here we used an extensive allelic series of mouse knockouts to show that the miR-196 family of miRNAs is essential both for properly patterning vertebral identity at different axial levels and for modulating the total number of vertebrae. All three miR-196 paralogs, 196a1, 196a2, and 196b, act redundantly to pattern the midthoracic region, whereas 196a2 and 196b have an additive role in controlling the number of rib-bearing vertebra and positioning of the sacrum. Independent of this, 196a1, 196a2, and 196b act redundantly to constrain total vertebral number. Loss of miR-196 leads to a collective up-regulation of numerous trunk Hox target genes with a concomitant delay in activation of caudal Hox genes, which are proposed to signal the end of axis extension. Additionally, we identified altered molecular signatures associated with the Wnt, Fgf, and Notch/segmentation pathways and demonstrate that miR-196 has the potential to regulate Wnt activity by multiple mechanisms. By feeding into, and thereby integrating, multiple genetic networks controlling vertebral number and identity, miR-196 is a critical player defining axial formulae.

AB - The Hox genes play a central role in patterning the embryonic anterior-to-posterior axis. An important function of Hox activity in vertebrates is the specification of different vertebral morphologies, with an additional role in axis elongation emerging. The miR-196 family of microRNAs (miRNAs) are predicted to extensively target Hox 3 UTRs, although the full extent to which miR-196 regulates Hox expression dynamics and influences mammalian development remains to be elucidated. Here we used an extensive allelic series of mouse knockouts to show that the miR-196 family of miRNAs is essential both for properly patterning vertebral identity at different axial levels and for modulating the total number of vertebrae. All three miR-196 paralogs, 196a1, 196a2, and 196b, act redundantly to pattern the midthoracic region, whereas 196a2 and 196b have an additive role in controlling the number of rib-bearing vertebra and positioning of the sacrum. Independent of this, 196a1, 196a2, and 196b act redundantly to constrain total vertebral number. Loss of miR-196 leads to a collective up-regulation of numerous trunk Hox target genes with a concomitant delay in activation of caudal Hox genes, which are proposed to signal the end of axis extension. Additionally, we identified altered molecular signatures associated with the Wnt, Fgf, and Notch/segmentation pathways and demonstrate that miR-196 has the potential to regulate Wnt activity by multiple mechanisms. By feeding into, and thereby integrating, multiple genetic networks controlling vertebral number and identity, miR-196 is a critical player defining axial formulae.

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U2 - 10.1073/pnas.1512655112

DO - 10.1073/pnas.1512655112

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JF - Proceedings of the National Academy of Sciences of the United States of America

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