TY - JOUR
T1 - Increased production of alkaline polygalacturonate lyase in the recombinant Pichia pastoris by controlling cell concentration during continuous culture
AU - Wang, Huilin
AU - Li, Jianghua
AU - Liu, Long
AU - Li, Xiaoman
AU - Jia, Dongxu
AU - Du, Guocheng
AU - Chen, Jian
AU - Song, Jiangning
PY - 2012
Y1 - 2012
N2 - Recombinant alkaline polygalacturonate lyase (PGL) production by recombinant Pichia pastoris GS115 was selected as a model to study as a continuous culture strategy for enhancing heterologous protein production based on controlling methanol feeding (CCCM culture) or on dual carbon source feeding (CCCD culture). Using the CCCM process with a dry cell weight of 75g/L regulated by controlling methanol concentration in the induction media, the final PGL activity was 441.9U/mL. The PGL productivity (Q(v)) and the average specific enzyme production rate (Q(x)) were 4.65UmL(-1)h(-1) and 84.5Ug(-1)h(-1), an increase of 42.1 and 191.2 , respectively, over what was achieved with traditional fed-batch culture with high cell density. The control strategies also reduced proteolytic degradation by 84.1 in the fermentation broth and increased cell viability by 12.2 .
AB - Recombinant alkaline polygalacturonate lyase (PGL) production by recombinant Pichia pastoris GS115 was selected as a model to study as a continuous culture strategy for enhancing heterologous protein production based on controlling methanol feeding (CCCM culture) or on dual carbon source feeding (CCCD culture). Using the CCCM process with a dry cell weight of 75g/L regulated by controlling methanol concentration in the induction media, the final PGL activity was 441.9U/mL. The PGL productivity (Q(v)) and the average specific enzyme production rate (Q(x)) were 4.65UmL(-1)h(-1) and 84.5Ug(-1)h(-1), an increase of 42.1 and 191.2 , respectively, over what was achieved with traditional fed-batch culture with high cell density. The control strategies also reduced proteolytic degradation by 84.1 in the fermentation broth and increased cell viability by 12.2 .
UR - http://www.sciencedirect.com/science/article/pii/S0960852412012059
U2 - 10.1016/j.biortech.2012.08.027
DO - 10.1016/j.biortech.2012.08.027
M3 - Article
SN - 0960-8524
VL - 124
SP - 338
EP - 346
JO - Bioresource Technology
JF - Bioresource Technology
ER -