Increased fucoxanthin in Chaetoceros calcitrans extract exacerbates apoptosis in liver cancer cells via multiple targeted cellular pathways

Su Chern Foo, Fatimah Md Yusoff, Mustapha Umar Imam, Jhi Biau Foo, Norsharina Ismail, Nur Hanisah Azmi, Yin Sim Tor, Nicholas M.H. Khong, Maznah Ismail

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    11 Citations (Scopus)

    Abstract

    In this study, anti-proliferative effects of C. calcitrans extract and its fucoxanthin rich fraction (FxRF) were assessed on human liver HepG2 cancer cell line. Efficacy from each extract was determined by cytotoxicity assay, morphological observation, and cell cycle analysis. Mechanisms of action observed were evaluated using multiplex gene expression analysis. Results showed that CME and FxRF induced cytotoxicity to HepG2 cells in a dose and time-dependent manner. FxRF (IC50: 18.89 μg.mL−1) was found to be significantly more potent than CME (IC50: 87.5 μg.mL−1) (p < 0.05). Gene expression studies revealed that anti-proliferative effects in treated cells by C. calcitrans extracts were mediated partly through the modulation of numerous genes involved in cell signaling (AKT1, ERK1/2, JNK), apoptosis (BAX, BID, Bcl-2, APAF, CYCS) and oxidative stress (SOD1, SOD2, CAT). Overall, C. calcitrans extracts demonstrated effective intervention against HepG2 cancer cells where enhanced apoptotic activities were observed with increased fucoxanthin content.

    Original languageEnglish
    Article numbere00296
    Number of pages11
    JournalBiotechnology Reports
    Volume21
    DOIs
    Publication statusPublished - Mar 2019

    Keywords

    • Apoptosis
    • Chaetoceros calcitrans
    • Fucoxanthin
    • Gene expression
    • Microalgae
    • Rich fraction

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