Increase in Dye: Dendrimer Ratio Decreases Cellular Uptake of Neutral Dendrimers in RAW Cells

Sriram Vaidyanathan, Milan Kaushik, Casey Dougherty, Rahul Rattan, Sascha N. Goonewardena, Mark M. Banaszak Holl, Janet Monano, Stassi DiMaggio

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3 Citations (Scopus)

Abstract

Neutral generation 3 poly(amidoamine) dendrimers were labeled with Oregon Green 488 (G3-OGn) to obtain materials with controlled fluorophore:dendrimer ratios (n = 1-2), a mixture containing mostly 3 dyes per dendrimer, a mixture containing primarily 4 or more dyes per dendrimer (n = 4+), and a stochastic mixture (n = 4avg). The UV absorbance of the dye conjugates increased linearly as n increased and the fluorescence emission decreased linearly as n increased. Cellular uptake was studied in RAW cells and HEK 293A cells as a function of the fluorophore:dendrimer ratio (n). The cellular uptake of G3-OGn (n = 3, 4+, 4avg) into RAW cells was significantly lower than G3-OGn (n = 1, 2). The uptake of G3-OGn (n = 3, 4+, 4avg) into HEK 293A cells was not significantly different from G3-OG1. Thus, the fluorophore:dendrimer ratio was observed to change the extent of uptake in the macrophage uptake mechanism but not in the HEK 293A cell. This difference in endocytosis indicates the presence of a pathway in the macrophage that is sensitive to hydrophobicity of the particle.

Original languageEnglish
Pages (from-to)1540-1545
Number of pages6
JournalACS Biomaterials Science & Engineering
Volume2
Issue number9
DOIs
Publication statusPublished - 12 Sep 2016
Externally publishedYes

Keywords

  • dye
  • fluorescence emission
  • HPLC
  • mammalian cell
  • PAMAM dendrimer

Cite this

Vaidyanathan, S., Kaushik, M., Dougherty, C., Rattan, R., Goonewardena, S. N., Banaszak Holl, M. M., Monano, J., & DiMaggio, S. (2016). Increase in Dye: Dendrimer Ratio Decreases Cellular Uptake of Neutral Dendrimers in RAW Cells. ACS Biomaterials Science & Engineering, 2(9), 1540-1545. https://doi.org/10.1021/acsbiomaterials.6b00308