Increase in Dye: Dendrimer Ratio Decreases Cellular Uptake of Neutral Dendrimers in RAW Cells

Sriram Vaidyanathan, Milan Kaushik, Casey Dougherty, Rahul Rattan, Sascha N. Goonewardena, Mark M. Banaszak Holl, Janet Monano, Stassi DiMaggio

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Neutral generation 3 poly(amidoamine) dendrimers were labeled with Oregon Green 488 (G3-OGn) to obtain materials with controlled fluorophore:dendrimer ratios (n = 1-2), a mixture containing mostly 3 dyes per dendrimer, a mixture containing primarily 4 or more dyes per dendrimer (n = 4+), and a stochastic mixture (n = 4avg). The UV absorbance of the dye conjugates increased linearly as n increased and the fluorescence emission decreased linearly as n increased. Cellular uptake was studied in RAW cells and HEK 293A cells as a function of the fluorophore:dendrimer ratio (n). The cellular uptake of G3-OGn (n = 3, 4+, 4avg) into RAW cells was significantly lower than G3-OGn (n = 1, 2). The uptake of G3-OGn (n = 3, 4+, 4avg) into HEK 293A cells was not significantly different from G3-OG1. Thus, the fluorophore:dendrimer ratio was observed to change the extent of uptake in the macrophage uptake mechanism but not in the HEK 293A cell. This difference in endocytosis indicates the presence of a pathway in the macrophage that is sensitive to hydrophobicity of the particle.

Original languageEnglish
Pages (from-to)1540-1545
Number of pages6
JournalACS Biomaterials Science and Engineering
Volume2
Issue number9
DOIs
Publication statusPublished - 12 Sep 2016
Externally publishedYes

Keywords

  • dye
  • fluorescence emission
  • HPLC
  • mammalian cell
  • PAMAM dendrimer

Cite this

Vaidyanathan, Sriram ; Kaushik, Milan ; Dougherty, Casey ; Rattan, Rahul ; Goonewardena, Sascha N. ; Banaszak Holl, Mark M. ; Monano, Janet ; DiMaggio, Stassi. / Increase in Dye : Dendrimer Ratio Decreases Cellular Uptake of Neutral Dendrimers in RAW Cells. In: ACS Biomaterials Science and Engineering. 2016 ; Vol. 2, No. 9. pp. 1540-1545.
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abstract = "Neutral generation 3 poly(amidoamine) dendrimers were labeled with Oregon Green 488 (G3-OGn) to obtain materials with controlled fluorophore:dendrimer ratios (n = 1-2), a mixture containing mostly 3 dyes per dendrimer, a mixture containing primarily 4 or more dyes per dendrimer (n = 4+), and a stochastic mixture (n = 4avg). The UV absorbance of the dye conjugates increased linearly as n increased and the fluorescence emission decreased linearly as n increased. Cellular uptake was studied in RAW cells and HEK 293A cells as a function of the fluorophore:dendrimer ratio (n). The cellular uptake of G3-OGn (n = 3, 4+, 4avg) into RAW cells was significantly lower than G3-OGn (n = 1, 2). The uptake of G3-OGn (n = 3, 4+, 4avg) into HEK 293A cells was not significantly different from G3-OG1. Thus, the fluorophore:dendrimer ratio was observed to change the extent of uptake in the macrophage uptake mechanism but not in the HEK 293A cell. This difference in endocytosis indicates the presence of a pathway in the macrophage that is sensitive to hydrophobicity of the particle.",
keywords = "dye, fluorescence emission, HPLC, mammalian cell, PAMAM dendrimer",
author = "Sriram Vaidyanathan and Milan Kaushik and Casey Dougherty and Rahul Rattan and Goonewardena, {Sascha N.} and {Banaszak Holl}, {Mark M.} and Janet Monano and Stassi DiMaggio",
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Vaidyanathan, S, Kaushik, M, Dougherty, C, Rattan, R, Goonewardena, SN, Banaszak Holl, MM, Monano, J & DiMaggio, S 2016, 'Increase in Dye: Dendrimer Ratio Decreases Cellular Uptake of Neutral Dendrimers in RAW Cells' ACS Biomaterials Science and Engineering, vol. 2, no. 9, pp. 1540-1545. https://doi.org/10.1021/acsbiomaterials.6b00308

Increase in Dye : Dendrimer Ratio Decreases Cellular Uptake of Neutral Dendrimers in RAW Cells. / Vaidyanathan, Sriram; Kaushik, Milan; Dougherty, Casey; Rattan, Rahul; Goonewardena, Sascha N.; Banaszak Holl, Mark M.; Monano, Janet; DiMaggio, Stassi.

In: ACS Biomaterials Science and Engineering, Vol. 2, No. 9, 12.09.2016, p. 1540-1545.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Increase in Dye

T2 - Dendrimer Ratio Decreases Cellular Uptake of Neutral Dendrimers in RAW Cells

AU - Vaidyanathan, Sriram

AU - Kaushik, Milan

AU - Dougherty, Casey

AU - Rattan, Rahul

AU - Goonewardena, Sascha N.

AU - Banaszak Holl, Mark M.

AU - Monano, Janet

AU - DiMaggio, Stassi

PY - 2016/9/12

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KW - fluorescence emission

KW - HPLC

KW - mammalian cell

KW - PAMAM dendrimer

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DO - 10.1021/acsbiomaterials.6b00308

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EP - 1545

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