TY - JOUR
T1 - Increase in Dye
T2 - Dendrimer Ratio Decreases Cellular Uptake of Neutral Dendrimers in RAW Cells
AU - Vaidyanathan, Sriram
AU - Kaushik, Milan
AU - Dougherty, Casey
AU - Rattan, Rahul
AU - Goonewardena, Sascha N.
AU - Banaszak Holl, Mark M.
AU - Monano, Janet
AU - DiMaggio, Stassi
PY - 2016/9/12
Y1 - 2016/9/12
N2 - Neutral generation 3 poly(amidoamine) dendrimers were labeled with Oregon Green 488 (G3-OGn) to obtain materials with controlled fluorophore:dendrimer ratios (n = 1-2), a mixture containing mostly 3 dyes per dendrimer, a mixture containing primarily 4 or more dyes per dendrimer (n = 4+), and a stochastic mixture (n = 4avg). The UV absorbance of the dye conjugates increased linearly as n increased and the fluorescence emission decreased linearly as n increased. Cellular uptake was studied in RAW cells and HEK 293A cells as a function of the fluorophore:dendrimer ratio (n). The cellular uptake of G3-OGn (n = 3, 4+, 4avg) into RAW cells was significantly lower than G3-OGn (n = 1, 2). The uptake of G3-OGn (n = 3, 4+, 4avg) into HEK 293A cells was not significantly different from G3-OG1. Thus, the fluorophore:dendrimer ratio was observed to change the extent of uptake in the macrophage uptake mechanism but not in the HEK 293A cell. This difference in endocytosis indicates the presence of a pathway in the macrophage that is sensitive to hydrophobicity of the particle.
AB - Neutral generation 3 poly(amidoamine) dendrimers were labeled with Oregon Green 488 (G3-OGn) to obtain materials with controlled fluorophore:dendrimer ratios (n = 1-2), a mixture containing mostly 3 dyes per dendrimer, a mixture containing primarily 4 or more dyes per dendrimer (n = 4+), and a stochastic mixture (n = 4avg). The UV absorbance of the dye conjugates increased linearly as n increased and the fluorescence emission decreased linearly as n increased. Cellular uptake was studied in RAW cells and HEK 293A cells as a function of the fluorophore:dendrimer ratio (n). The cellular uptake of G3-OGn (n = 3, 4+, 4avg) into RAW cells was significantly lower than G3-OGn (n = 1, 2). The uptake of G3-OGn (n = 3, 4+, 4avg) into HEK 293A cells was not significantly different from G3-OG1. Thus, the fluorophore:dendrimer ratio was observed to change the extent of uptake in the macrophage uptake mechanism but not in the HEK 293A cell. This difference in endocytosis indicates the presence of a pathway in the macrophage that is sensitive to hydrophobicity of the particle.
KW - dye
KW - fluorescence emission
KW - HPLC
KW - mammalian cell
KW - PAMAM dendrimer
UR - http://www.scopus.com/inward/record.url?scp=85000702073&partnerID=8YFLogxK
U2 - 10.1021/acsbiomaterials.6b00308
DO - 10.1021/acsbiomaterials.6b00308
M3 - Article
C2 - 28286863
AN - SCOPUS:85000702073
SN - 2373-9878
VL - 2
SP - 1540
EP - 1545
JO - ACS Biomaterials Science & Engineering
JF - ACS Biomaterials Science & Engineering
IS - 9
ER -