In vivo testing of 177Lu-labelled anti-PSMA antibody as a new radioimmunotherapeutic agent against prostate cancer

Martin Behe, Karen Alt, Friederike Deininger, Patrick Bühler, Ulrich Wetterauer, Wolfgang A Weber, Ursula Elsässer-Beile, Philipp Wolf

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17 Citations (Scopus)

Abstract

Aim: The goal of the present study was to test the 177Lu- labelled anti-PSMA monoclonal antibody 3/F11 (177Lu-DOTA-3/F11) as a new radioimmunotherapeutic agent in a prostate cancer SCID mouse xenograft model. Materials and Methods: The mAb 3/FU was 177Lu labelled using 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid (DOTA) as chelating agent. DOTA-3/F11 was tested for cell binding and serum immunoreactivity by flow cytometry. The biodistribution and the therapeutic efficacy of 177Lu-DOTA-3/F11 in mice bearing PSMA-positive C4-2 prostate cancer xenografts were evaluated. Results: 3/F11 and DOTA-3/F11 showed high and specific cell binding and similar serum half-lives of approximately seven days. Biodistribution studies revealed an increasing tumour uptake of 177Lu DOTA-3/F11 over time with maximum tumour-to-muscle and tumour-to-blood ratios after 72 h. A single dose of 1 MBq 177Lu-DOTA-3/F11 inhibited tumour growth and prolonged survival. Conclusion: This study indicated that 177Lu-DOTA-3/F11 may be a suitable radioimmunotherapeutic agent for the treatment of prostate cancer.

Original languageEnglish
Pages (from-to)55-59
Number of pages5
JournalIn Vivo
Volume25
Issue number1
Publication statusPublished - Jan 2011
Externally publishedYes

Keywords

  • Lu-DOTA-3/F11
  • Monoclonal antibody
  • Prostate cancer
  • PSMA
  • Radioimmunotherapy

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