In-vivo single neuron axotomy triggers axon regeneration to restore synaptic density in specific cortical circuits

A J Canty, Lieven Huang, J S Jackson, G E Little, G Knott, Bohumil Maco, V De Paola

Research output: Contribution to journalArticleResearchpeer-review

44 Citations (Scopus)

Abstract

To what extent, how and when axons respond to injury in the highly interconnected grey matter is poorly understood. Here we use two-photon imaging and focused ion beam-scanning electron microscopy to explore, at synaptic resolution, the regrowth capacity of several neuronal populations in the intact brain. Time-lapse analysis of >100 individually ablated axons for periods of up to a year reveals a surprising inability to regenerate even in a glial scar-free environment. However, depending on cell type some axons spontaneously extend for distances unseen in the unlesioned adult cortex and at maximum speeds comparable to peripheral nerve regeneration. Regrowth follows a distinct pattern from developmental axon growth. Remarkably, although never reconnecting to the original targets, axons consistently form new boutons at comparable prelesion synaptic densities, implying the existence of intrinsic homeostatic programmes, which regulate synaptic numbers on regenerating axons. Our results may help guide future clinical investigations to promote functional axon regeneration.
Original languageEnglish
Pages (from-to)1 - 10
Number of pages10
JournalNature Communications
Volume4
Issue numberArt. ID: 2038
DOIs
Publication statusPublished - 2013

Cite this

Canty, A. J., Huang, L., Jackson, J. S., Little, G. E., Knott, G., Maco, B., & De Paola, V. (2013). In-vivo single neuron axotomy triggers axon regeneration to restore synaptic density in specific cortical circuits. Nature Communications, 4(Art. ID: 2038), 1 - 10. https://doi.org/10.1038/ncomms3038
Canty, A J ; Huang, Lieven ; Jackson, J S ; Little, G E ; Knott, G ; Maco, Bohumil ; De Paola, V. / In-vivo single neuron axotomy triggers axon regeneration to restore synaptic density in specific cortical circuits. In: Nature Communications. 2013 ; Vol. 4, No. Art. ID: 2038. pp. 1 - 10.
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Canty, AJ, Huang, L, Jackson, JS, Little, GE, Knott, G, Maco, B & De Paola, V 2013, 'In-vivo single neuron axotomy triggers axon regeneration to restore synaptic density in specific cortical circuits', Nature Communications, vol. 4, no. Art. ID: 2038, pp. 1 - 10. https://doi.org/10.1038/ncomms3038

In-vivo single neuron axotomy triggers axon regeneration to restore synaptic density in specific cortical circuits. / Canty, A J; Huang, Lieven; Jackson, J S; Little, G E; Knott, G; Maco, Bohumil; De Paola, V.

In: Nature Communications, Vol. 4, No. Art. ID: 2038, 2013, p. 1 - 10.

Research output: Contribution to journalArticleResearchpeer-review

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