In vivo infection model of severe influenza a virus

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5 Citations (Scopus)


The lung is constantly exposed to both environmental and microbial challenge. As a “contained” organ, it also constitutes an excellent “self-contained” tissue to examine inflammatory responses and cellular infiltration into a diseased organ. Influenza A virus (IAV) causes both mild and severe inflammation that is strain specific following infection of the lung epithelium that spreads to other cells of the lung environment. Here, we describe a method of intranasal inoculation of the lung with IAV that can be used as a preclinical model of infection. Mice can be monitored for clinical signs of infection and tissue and lung fluid collected for further analysis to dissect the immunological consequences of IAV infection. Importantly, this method can be modified to introduce other pathogens, therapies and environmental stimuli to examine immune responses in the lung.

Original languageEnglish
Title of host publicationInflammation and Cancer
Subtitle of host publicationMethods and Protocols
Place of PublicationNew York, NY
PublisherHumana Press
Number of pages9
ISBN (Electronic)9781493975686
ISBN (Print)9781493975679
Publication statusPublished - 1 Jan 2018

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029


  • Anesthetic
  • Bronchoalveolar lavage (BAL)
  • Clinical signs of disease
  • HKx31
  • Influenza A virus
  • Intranasal
  • PR8

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