In vivo imaging and tracking of individual nanodiamonds in Drosophila melanogaster embryos

David A. Simpson; Amelia J. Thompson; Mark Kowarsky; Nida F. Zeeshan; Michael S.J. Barson; Liam T. Hall; Yan Yan; Stefan Kaufmann; Brett C. Johnson; Takeshi Ohshima; Frank Caruso; Robert E. Scholten; Robert B. Saint; Michael J. Murray; Lloyd C.L. Hollenberg

doi:10.1364/BOE.5.001250

In vivo imaging and tracking of individual nanodiamonds in Drosophila melanogaster embryos

David A. Simpson, Amelia J. Thompson, Mark Kowarsky, Nida F. Zeeshan, Michael S.J. Barson, Liam T. Hall, Yan Yan, Stefan Kaufmann, Brett C. Johnson, Takeshi Ohshima, Frank Caruso, Robert E. Scholten, Robert B. Saint, Michael J. Murray, Lloyd C.L. Hollenberg

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Abstract

In this work, we incorporate and image individual fluorescent nanodiamonds in the powerful genetic model system Drosophila melanogaster. Fluorescence correlation spectroscopy and wide-field imaging techniques are applied to individual fluorescent nanodiamonds in blastoderm cells during stage 5 of development, up to a depth of 40 μm. The majority of nanodiamonds in the blastoderm cells during cellularization exhibit free diffusion with an average diffusion coefficient of (6 ± 3) × 10^-3 μm²/s, (mean ± SD). Driven motion in the blastoderm cells was also observed with an average velocity of 0.13 ± 0.10 μm/s (mean ± SD) μm/s and an average applied force of 0.07 ± 0.05 pN (mean ± SD). Nanodiamonds in the periplasm between the nuclei and yolk were also found to undergo free diffusion with a significantly larger diffusion coefficient of (63 ± 35) × 10^-3 μm²/s (mean ± SD). Driven motion in this region exhibited similar average velocities and applied forces compared to the blastoderm cells indicating the transport dynamics in the two cytoplasmic regions are analogous.

Original language	English
Pages (from-to)	1250-1261
Number of pages	12
Journal	Biomedical Optics Express
Volume	5
Issue number	4
DOIs	https://doi.org/10.1364/BOE.5.001250
Publication status	Published - 1 Apr 2014
Externally published	Yes

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Simpson, D. A., Thompson, A. J., Kowarsky, M., Zeeshan, N. F., Barson, M. S. J., Hall, L. T., Yan, Y., Kaufmann, S., Johnson, B. C., Ohshima, T., Caruso, F., Scholten, R. E., Saint, R. B., Murray, M. J., & Hollenberg, L. C. L. (2014). In vivo imaging and tracking of individual nanodiamonds in Drosophila melanogaster embryos. Biomedical Optics Express, 5(4), 1250-1261. https://doi.org/10.1364/BOE.5.001250

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title = "In vivo imaging and tracking of individual nanodiamonds in Drosophila melanogaster embryos",

abstract = "In this work, we incorporate and image individual fluorescent nanodiamonds in the powerful genetic model system Drosophila melanogaster. Fluorescence correlation spectroscopy and wide-field imaging techniques are applied to individual fluorescent nanodiamonds in blastoderm cells during stage 5 of development, up to a depth of 40 μm. The majority of nanodiamonds in the blastoderm cells during cellularization exhibit free diffusion with an average diffusion coefficient of (6 ± 3) × 10-3 μm2/s, (mean ± SD). Driven motion in the blastoderm cells was also observed with an average velocity of 0.13 ± 0.10 μm/s (mean ± SD) μm/s and an average applied force of 0.07 ± 0.05 pN (mean ± SD). Nanodiamonds in the periplasm between the nuclei and yolk were also found to undergo free diffusion with a significantly larger diffusion coefficient of (63 ± 35) × 10-3 μm2/s (mean ± SD). Driven motion in this region exhibited similar average velocities and applied forces compared to the blastoderm cells indicating the transport dynamics in the two cytoplasmic regions are analogous.",

author = "Simpson, {David A.} and Thompson, {Amelia J.} and Mark Kowarsky and Zeeshan, {Nida F.} and Barson, {Michael S.J.} and Hall, {Liam T.} and Yan Yan and Stefan Kaufmann and Johnson, {Brett C.} and Takeshi Ohshima and Frank Caruso and Scholten, {Robert E.} and Saint, {Robert B.} and Murray, {Michael J.} and Hollenberg, {Lloyd C.L.}",

year = "2014",

month = apr,

day = "1",

doi = "10.1364/BOE.5.001250",

language = "English",

volume = "5",

pages = "1250--1261",

journal = "Biomedical Optics Express",

issn = "2156-7085",

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Simpson, DA, Thompson, AJ, Kowarsky, M, Zeeshan, NF, Barson, MSJ, Hall, LT, Yan, Y, Kaufmann, S, Johnson, BC, Ohshima, T, Caruso, F, Scholten, RE, Saint, RB, Murray, MJ & Hollenberg, LCL 2014, 'In vivo imaging and tracking of individual nanodiamonds in Drosophila melanogaster embryos', Biomedical Optics Express, vol. 5, no. 4, pp. 1250-1261. https://doi.org/10.1364/BOE.5.001250

TY - JOUR

T1 - In vivo imaging and tracking of individual nanodiamonds in Drosophila melanogaster embryos

AU - Simpson, David A.

AU - Thompson, Amelia J.

AU - Kowarsky, Mark

AU - Zeeshan, Nida F.

AU - Barson, Michael S.J.

AU - Hall, Liam T.

AU - Yan, Yan

AU - Kaufmann, Stefan

AU - Johnson, Brett C.

AU - Ohshima, Takeshi

AU - Caruso, Frank

AU - Scholten, Robert E.

AU - Saint, Robert B.

AU - Murray, Michael J.

AU - Hollenberg, Lloyd C.L.

PY - 2014/4/1

Y1 - 2014/4/1

N2 - In this work, we incorporate and image individual fluorescent nanodiamonds in the powerful genetic model system Drosophila melanogaster. Fluorescence correlation spectroscopy and wide-field imaging techniques are applied to individual fluorescent nanodiamonds in blastoderm cells during stage 5 of development, up to a depth of 40 μm. The majority of nanodiamonds in the blastoderm cells during cellularization exhibit free diffusion with an average diffusion coefficient of (6 ± 3) × 10-3 μm2/s, (mean ± SD). Driven motion in the blastoderm cells was also observed with an average velocity of 0.13 ± 0.10 μm/s (mean ± SD) μm/s and an average applied force of 0.07 ± 0.05 pN (mean ± SD). Nanodiamonds in the periplasm between the nuclei and yolk were also found to undergo free diffusion with a significantly larger diffusion coefficient of (63 ± 35) × 10-3 μm2/s (mean ± SD). Driven motion in this region exhibited similar average velocities and applied forces compared to the blastoderm cells indicating the transport dynamics in the two cytoplasmic regions are analogous.

AB - In this work, we incorporate and image individual fluorescent nanodiamonds in the powerful genetic model system Drosophila melanogaster. Fluorescence correlation spectroscopy and wide-field imaging techniques are applied to individual fluorescent nanodiamonds in blastoderm cells during stage 5 of development, up to a depth of 40 μm. The majority of nanodiamonds in the blastoderm cells during cellularization exhibit free diffusion with an average diffusion coefficient of (6 ± 3) × 10-3 μm2/s, (mean ± SD). Driven motion in the blastoderm cells was also observed with an average velocity of 0.13 ± 0.10 μm/s (mean ± SD) μm/s and an average applied force of 0.07 ± 0.05 pN (mean ± SD). Nanodiamonds in the periplasm between the nuclei and yolk were also found to undergo free diffusion with a significantly larger diffusion coefficient of (63 ± 35) × 10-3 μm2/s (mean ± SD). Driven motion in this region exhibited similar average velocities and applied forces compared to the blastoderm cells indicating the transport dynamics in the two cytoplasmic regions are analogous.

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JO - Biomedical Optics Express

JF - Biomedical Optics Express

SN - 2156-7085

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ER -

In vivo imaging and tracking of individual nanodiamonds in Drosophila melanogaster embryos

Abstract

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