In vitro screening of dry fruit extracts of Piper attenuatum for antioxidant and anticancer activity

Renu Ohlyan; Ajit Kandale; Girdhar Singh Deora; Vandana Rathore; Jagbir Chahal

doi:10.1007/s00044-012-0122-y

In vitro screening of dry fruit extracts of Piper attenuatum for antioxidant and anticancer activity

Renu Ohlyan, Ajit Kandale, Girdhar Singh Deora, Vandana Rathore, Jagbir Chahal

Research output: Contribution to journal › Article › Research › peer-review

7 Citations (Scopus)

Abstract

Indian traditional medicinal plant Piper attenuatum (Buch-Ham) has been investigated for its antioxidant and anticancer activity. Three extracts were prepared using ethyl acetate, ethanol and methanol. In vitro antioxidant activity was performed by ABTS {2,2′-azino-bis(3-ethylbenzothiazoline-6- sulphonic acid)} free radical scavenging method. All three extracts reduced the free radicals produced by ABTS in a concentration-dependent manner which could be compared to the standard (gallic acid). Invitro anticancer activity of all extracts was carried out by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide) assay method against MCF7 (Michigan Cancer Foundation-7) cell lines. None of the extract showed anticancer activity when compared with the standard (mitomycin C) indicating that P. attenuatum is deprived of antiproliferative or cytotoxic components.

Original language	English
Pages (from-to)	1365-1370
Number of pages	6
Journal	Medicinal Chemistry Research
Volume	22
Issue number	3
DOIs	https://doi.org/10.1007/s00044-012-0122-y
Publication status	Published - Mar 2013
Externally published	Yes

Keywords

ABTS assay
Anticancer
Antioxidant
MCF7 cell lines
Piper attenuatum

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1007/s00044-012-0122-y

Cite this

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title = "In vitro screening of dry fruit extracts of Piper attenuatum for antioxidant and anticancer activity",

abstract = "Indian traditional medicinal plant Piper attenuatum (Buch-Ham) has been investigated for its antioxidant and anticancer activity. Three extracts were prepared using ethyl acetate, ethanol and methanol. In vitro antioxidant activity was performed by ABTS {2,2′-azino-bis(3-ethylbenzothiazoline-6- sulphonic acid)} free radical scavenging method. All three extracts reduced the free radicals produced by ABTS in a concentration-dependent manner which could be compared to the standard (gallic acid). Invitro anticancer activity of all extracts was carried out by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide) assay method against MCF7 (Michigan Cancer Foundation-7) cell lines. None of the extract showed anticancer activity when compared with the standard (mitomycin C) indicating that P. attenuatum is deprived of antiproliferative or cytotoxic components. ",

keywords = "ABTS assay, Anticancer, Antioxidant, MCF7 cell lines, Piper attenuatum",

author = "Renu Ohlyan and Ajit Kandale and Deora, {Girdhar Singh} and Vandana Rathore and Jagbir Chahal",

year = "2013",

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journal = "Medicinal Chemistry Research",

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AU - Ohlyan, Renu

AU - Kandale, Ajit

AU - Deora, Girdhar Singh

AU - Rathore, Vandana

AU - Chahal, Jagbir

PY - 2013/3

Y1 - 2013/3

N2 - Indian traditional medicinal plant Piper attenuatum (Buch-Ham) has been investigated for its antioxidant and anticancer activity. Three extracts were prepared using ethyl acetate, ethanol and methanol. In vitro antioxidant activity was performed by ABTS {2,2′-azino-bis(3-ethylbenzothiazoline-6- sulphonic acid)} free radical scavenging method. All three extracts reduced the free radicals produced by ABTS in a concentration-dependent manner which could be compared to the standard (gallic acid). Invitro anticancer activity of all extracts was carried out by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide) assay method against MCF7 (Michigan Cancer Foundation-7) cell lines. None of the extract showed anticancer activity when compared with the standard (mitomycin C) indicating that P. attenuatum is deprived of antiproliferative or cytotoxic components.

AB - Indian traditional medicinal plant Piper attenuatum (Buch-Ham) has been investigated for its antioxidant and anticancer activity. Three extracts were prepared using ethyl acetate, ethanol and methanol. In vitro antioxidant activity was performed by ABTS {2,2′-azino-bis(3-ethylbenzothiazoline-6- sulphonic acid)} free radical scavenging method. All three extracts reduced the free radicals produced by ABTS in a concentration-dependent manner which could be compared to the standard (gallic acid). Invitro anticancer activity of all extracts was carried out by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide) assay method against MCF7 (Michigan Cancer Foundation-7) cell lines. None of the extract showed anticancer activity when compared with the standard (mitomycin C) indicating that P. attenuatum is deprived of antiproliferative or cytotoxic components.

KW - ABTS assay

KW - Anticancer

KW - Antioxidant

KW - MCF7 cell lines

KW - Piper attenuatum

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ER -

In vitro screening of dry fruit extracts of Piper attenuatum for antioxidant and anticancer activity

Abstract

Keywords

UN SDGs

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