In vitro screening of dry fruit extracts of Piper attenuatum for antioxidant and anticancer activity

Renu Ohlyan, Ajit Kandale, Girdhar Singh Deora, Vandana Rathore, Jagbir Chahal

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Indian traditional medicinal plant Piper attenuatum (Buch-Ham) has been investigated for its antioxidant and anticancer activity. Three extracts were prepared using ethyl acetate, ethanol and methanol. In vitro antioxidant activity was performed by ABTS {2,2′-azino-bis(3-ethylbenzothiazoline-6- sulphonic acid)} free radical scavenging method. All three extracts reduced the free radicals produced by ABTS in a concentration-dependent manner which could be compared to the standard (gallic acid). Invitro anticancer activity of all extracts was carried out by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide) assay method against MCF7 (Michigan Cancer Foundation-7) cell lines. None of the extract showed anticancer activity when compared with the standard (mitomycin C) indicating that P. attenuatum is deprived of antiproliferative or cytotoxic components. 

Original languageEnglish
Pages (from-to)1365-1370
Number of pages6
JournalMedicinal Chemistry Research
Issue number3
Publication statusPublished - Mar 2013
Externally publishedYes


  • ABTS assay
  • Anticancer
  • Antioxidant
  • MCF7 cell lines
  • Piper attenuatum

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