In vitro propagation of cauliflower, Brassica oleracea var. botrytis for hybrid seed production

Prem L. Bhalla, Nicole De Weerd

Research output: Contribution to journalArticleResearchpeer-review

21 Citations (Scopus)


Methods for obtaining heterotic F1 and maintaining purebred lines for breeding of Brassica oleracea are limited by absence of male sterile lines and occurrence of inbreeding depression, respectively. The use of vegetative (stem, petiole, leaf, leaf rib) and floral (peduncle, pedicel, flower bud, curd) explants of cauliflower to regenerate purebred lines for crossing were examined. Of four growth regulator treatments and explant types used, best results were obtained with curd explants on MS medium with 6-benzyladenine (cytokinin) and gibberellic acid. Although 6-benzyladenine alone promoted formation of shoots in floral explants, both 6-benzyladenine and α-napthaleneacetic acid were required for vegetative explants. Use of α-napthaleneacetic acid, however, often increased callus formation. These culture techniques to maintain purebred regenerated plants will complement newly-derived nuclear-based male sterile lines obtained by the introduction of antisense copies of the gene BcpI, which is required for pollen fertility.

Original languageEnglish
Pages (from-to)89-95
Number of pages7
JournalPlant Cell, Tissue and Organ Culture
Issue number2
Publication statusPublished - 1 Jan 1999
Externally publishedYes


  • Brassicaceae
  • In vitro culture
  • Plant regeneration

Cite this