In vitro primary responses of human T cells to soluble protein antigens

Magdalena Plebanski, Sally S. Burtles

Research output: Contribution to journalArticleResearchpeer-review

37 Citations (Scopus)


The optimum culture conditions to support in vitro proliferative responses to conventional soluble protein antigens by human CD4+ T cells from healthy non-immunised donors have been determined. These responses were blocked by anti-HLA-DR monoclonal antibodies (mAbs) and were strictly dependent on the presence of antigen-presenting cells (APCs). Primary responses showed a reproducible pattern of proliferation kinetics which distinguished them from secondary in vitro T cell reactions. T cells specific for the sensitising antigen were recovered from primary cultures.

Original languageEnglish
Pages (from-to)15-25
Number of pages11
JournalJournal of Immunological Methods
Issue number1
Publication statusPublished - 29 Mar 1994
Externally publishedYes


  • Conventional soluble antigen
  • Naive human T cell

Cite this