In vitro differentiation of pluripotent stem cells towards either forebrain GABAergic or midbrain dopaminergic neurons

Brigham J Hartley, Bradley Watmuff, Cameron P J Hunt, John M Haynes, Colin W Pouton

Research output: Chapter in Book/Report/Conference proceedingChapter (Book)Researchpeer-review

Abstract

There is considerable interest in the derivation of neuronal cells from pluripotent stem cells (PSCs). Cells produced in this way have potential uses in the treatment of neurodegenerative diseases, drug discovery studies, or for basic research in neuroscience. Here, we describe protocols for the directed differentiation of forebrain GABAergic and midbrain dopaminergic neurons, using murine and human PSCs.

Original languageEnglish
Title of host publicationNeural Stem Cell Assays
EditorsNavjot Kaur, Mohan C Vemuri
Place of PublicationHoboken NJ USA
PublisherJohn Wiley & Sons
Pages91-99
Number of pages9
ISBN (Electronic)9781118308295
ISBN (Print)9781118308257
DOIs
Publication statusPublished - 30 Jan 2015

Keywords

  • Directed differentiation
  • Dopaminergic neurons
  • GABAergic neurons
  • Human pluripotent stem cells
  • Mouse pluripotent stem cells
  • Neural differentiation

Cite this

Hartley, B. J., Watmuff, B., Hunt, C. P. J., Haynes, J. M., & Pouton, C. W. (2015). In vitro differentiation of pluripotent stem cells towards either forebrain GABAergic or midbrain dopaminergic neurons. In N. Kaur, & M. C. Vemuri (Eds.), Neural Stem Cell Assays (pp. 91-99). John Wiley & Sons. https://doi.org/10.1002/9781118308295.ch10