In vitro biosynthesis of ring-extended cyclosporins

A. Lawen, R. Traber, R. Reuille, M. Ponelle

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Cyclosporin synthetase, a multifunctional polypeptide, catalyses the biosynthesis of the set of natural cyclosporins. We report that this enzyme is also capable of introducing β-alanine into position 7 or 8 of the ring instead of the α-alanines present at these positions in cyclosporin A. This leads to 34-membered rings in contrast to the 33-membered ring of the cycloundecapeptide cyclosporin A. Both [βAla7]CyA and [βAla8](CyA show immunosuppressive activity. The cyclosporin synthetase-related enzyme peptolide SDZ 214-103 synthetase, on the other hand, does not incorporate either β-alanine into position 7 or β-hydroxy acids into position 8, confirming the previously described higher substrate specificity of this enzyme compared with cyclosporin synthetase

Original languageEnglish
Pages (from-to)395-399
Number of pages5
JournalBiochemical Journal
Issue number2
Publication statusPublished - 1 Jan 1994

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